Analytical Data
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基因名
EEFSEC
- Application
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别名
EEFSEC; SELB; Selenocysteine-specific elongation factor; Elongation factor sec; Eukaryotic elongation factor; selenocysteine-tRNA-specific
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P57772
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表达区间
1-569aa
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氨基酸序列
MASCSTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTHKRMVQSP
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分子量
88.99 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EEFSEC, or Eukaryotic Elongation Factor Sec, is a pivotal protein involved in the translation process, specifically in the synthesis of selenoproteins. Selenoproteins contain the rare amino acid selenocysteine, which is essential for various biological functions, including antioxidant defense and redox signaling. The unique incorporation of selenocysteine into proteins is facilitated by the EEFSEC, which assists in recognizing the UGA codon, traditionally a stop signal in the genetic code, allowing it to code for selenocysteine instead. Research on EEFSEC has gained momentum due to its implications in understanding not only protein synthesis but also the broader physiological roles of selenoproteins in health and disease. Misregulation of selenoprotein synthesis is linked to various clinical conditions, including cancer and neurodegenerative diseases, making EEFSEC a potential target for therapeutic strategies. The study of EEFSEC involves a combination of structural biology, biochemistry, and molecular genetics, providing insights into its function and mechanisms. As researchers develop recombinant forms of EEFSEC, they aim to elucidate its interactions and regulatory pathways, paving the way for advancements in both basic and applied sciences. This research not only enhances our understanding of fundamental biological processes but also opens avenues for novel interventions in diseases where selenoprotein synthesis is compromised.
