Analytical Data
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Gene name
EEFSEC
- Application
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Alternative Names
EEFSEC; SELB; Selenocysteine-specific elongation factor; Elongation factor sec; Eukaryotic elongation factor; selenocysteine-tRNA-specific
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P57772
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Expression Region
1-569aa
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AA Sequence
MASCSTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTHKRMVQSP
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Molecular Weight
88.99 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EEFSEC, or Eukaryotic Elongation Factor Sec, is a pivotal protein involved in the translation process, specifically in the synthesis of selenoproteins. Selenoproteins contain the rare amino acid selenocysteine, which is essential for various biological functions, including antioxidant defense and redox signaling. The unique incorporation of selenocysteine into proteins is facilitated by the EEFSEC, which assists in recognizing the UGA codon, traditionally a stop signal in the genetic code, allowing it to code for selenocysteine instead. Research on EEFSEC has gained momentum due to its implications in understanding not only protein synthesis but also the broader physiological roles of selenoproteins in health and disease. Misregulation of selenoprotein synthesis is linked to various clinical conditions, including cancer and neurodegenerative diseases, making EEFSEC a potential target for therapeutic strategies. The study of EEFSEC involves a combination of structural biology, biochemistry, and molecular genetics, providing insights into its function and mechanisms. As researchers develop recombinant forms of EEFSEC, they aim to elucidate its interactions and regulatory pathways, paving the way for advancements in both basic and applied sciences. This research not only enhances our understanding of fundamental biological processes but also opens avenues for novel interventions in diseases where selenoprotein synthesis is compromised.
