Analytical Data
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Gene name
EDG8
- Application
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Alternative Names
S1PR5; EDG8; Sphingosine 1-phosphate receptor 5; S1P receptor 5; S1P5; Endothelial differentiation G-protein-coupled receptor 8; Sphingosine 1-phosphate receptor Edg-8; S1P receptor Edg-8
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H228
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Expression Region
1-398aa
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AA Sequence
MESGLLRPAPVSEVIVLHYNYTGKLRGARYQPGAGLRADAVVCLAVCAFIVLENLAVLLVLGRHPRFHAPMFLLLGSLTLSDLLAGAAYAANILLSGPLTLKLSPALWFAREGGVFVALTASVLSLLAIALERSLTMARRGPAPVSSRGRTLAMAAAAWGVSLLLGLLPALGWNCLGRLDACSTVLPLYAKAYVLFCVLAFVGILAAICALYARIYCQVRANARRLPARPGTAGTTSTRARRKPRSLALLRTLSVVLLAFVACWGPLFLLLLLDVACPARTCPVLLQADPFLGLAMANSLLNPIIYTLTNRDLRHALLRLVCCGRHSCGRDPSGSQQSASAAEASGGLRRCLPPGLDGSFSGSERSSPQRDGLDTSGSTGSPGAPTAARTLVSEPAAD
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Molecular Weight
68.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The EDG8 protein, a member of the endothelial differentiation gene (EDG) family, plays a crucial role in various cellular functions, including cell proliferation, migration, and angiogenesis, primarily through its activity as a receptor for lysophosphatidic acid (LPA). Research into EDG8 has gained attention due to its implications in several physiological and pathological processes, such as cardiovascular diseases, cancer progression, and neurological disorders. The EDG8 receptor is known to interact with several signaling pathways, including those involving G proteins, which further mediate cellular responses to environmental stimuli. Understanding the structural and functional characteristics of the EDG8 protein is essential for uncovering its potential as a therapeutic target. Studies have also explored the recombinant expression of EDG8 to facilitate the analysis of its biological activities, receptor-ligand interactions, and the development of novel ligands or antagonists. This research is particularly important for identifying molecular mechanisms that underlie EDG8-related diseases and for developing targeted therapies that could modulate its activity in pathological conditions. Advances in techniques such as crystallography and cryo-electron microscopy are enhancing our understanding of EDG8's structure and function, paving the way for potential applications in drug design and personalized medicine. Overall, the study of EDG8 and its recombinant protein serves as a promising area for further exploration, with implications that extend across various fields of biomedical research.
