Analytical Data
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基因名
EDG8
- Application
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别名
S1PR5; EDG8; Sphingosine 1-phosphate receptor 5; S1P receptor 5; S1P5; Endothelial differentiation G-protein-coupled receptor 8; Sphingosine 1-phosphate receptor Edg-8; S1P receptor Edg-8
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H228
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表达区间
1-398aa
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氨基酸序列
MESGLLRPAPVSEVIVLHYNYTGKLRGARYQPGAGLRADAVVCLAVCAFIVLENLAVLLVLGRHPRFHAPMFLLLGSLTLSDLLAGAAYAANILLSGPLTLKLSPALWFAREGGVFVALTASVLSLLAIALERSLTMARRGPAPVSSRGRTLAMAAAAWGVSLLLGLLPALGWNCLGRLDACSTVLPLYAKAYVLFCVLAFVGILAAICALYARIYCQVRANARRLPARPGTAGTTSTRARRKPRSLALLRTLSVVLLAFVACWGPLFLLLLLDVACPARTCPVLLQADPFLGLAMANSLLNPIIYTLTNRDLRHALLRLVCCGRHSCGRDPSGSQQSASAAEASGGLRRCLPPGLDGSFSGSERSSPQRDGLDTSGSTGSPGAPTAARTLVSEPAAD
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分子量
68.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The EDG8 protein, a member of the endothelial differentiation gene (EDG) family, plays a crucial role in various cellular functions, including cell proliferation, migration, and angiogenesis, primarily through its activity as a receptor for lysophosphatidic acid (LPA). Research into EDG8 has gained attention due to its implications in several physiological and pathological processes, such as cardiovascular diseases, cancer progression, and neurological disorders. The EDG8 receptor is known to interact with several signaling pathways, including those involving G proteins, which further mediate cellular responses to environmental stimuli. Understanding the structural and functional characteristics of the EDG8 protein is essential for uncovering its potential as a therapeutic target. Studies have also explored the recombinant expression of EDG8 to facilitate the analysis of its biological activities, receptor-ligand interactions, and the development of novel ligands or antagonists. This research is particularly important for identifying molecular mechanisms that underlie EDG8-related diseases and for developing targeted therapies that could modulate its activity in pathological conditions. Advances in techniques such as crystallography and cryo-electron microscopy are enhancing our understanding of EDG8's structure and function, paving the way for potential applications in drug design and personalized medicine. Overall, the study of EDG8 and its recombinant protein serves as a promising area for further exploration, with implications that extend across various fields of biomedical research.
