Ipodix Yeast Expression System: Innovative Solutions for High-Efficiency Eukaryotic Protein Production
I. Technical Advantages: Six Core Values of the Yeast System
Ipodix has developed the next-generation YeastXpress™ technology platform based on Pichia pastoris and Saccharomyces cerevisiae dual systems, overcoming traditional expression limitations to achieve high-yield, cost-effective eukaryotic protein production.
1. High-Efficiency Expression
● Secretory expression yields of 2–10 g/L (5–20x higher than mammalian systems)
● Compatible with proteins ranging from 10 kDa to 300 kDa
2. Precision Post-Translational Modifications
● Glycosylation similarity to HEK293 system: 85%
● Disulfide bond accuracy >95%, phosphorylation site precision >90%
3. Cost-Effective Production
● Media costs reduced to 1/8 of mammalian cell systems
● Single-batch output in 30L fermenters meets kilogram-scale demands
4. Flexible Compatibility
● Supports both intracellular and secretory expression modes
● Multi-copy tandem expression vector construction
5. Stringent Quality Control
● Endotoxin levels <0.05 EU/μg (injection-grade standard)
● Batch-specific glycan analysis reports (MALDI-TOF verified)
6. Rapid Delivery
● Gene-to-purified protein in as little as 4 weeks
● 72-hour expedited channel for urgent projects
II. Service Workflow: End-to-End Technical Support with Standardized Operations
Ipodix’s integrated service system ensures an industry-leading project success rate of >90%:
Stage 1: Gene Construction & Optimization (1–2 weeks)
• Codon optimization tailored for 8 yeast strain preferences
• Promoter library screening (AOX1, GAP, FLD, etc.)
Stage 2: Strain Engineering (2–3 weeks)
• Multi-copy integration technology (1–12 copies optional)
• Custom protease-deficient strain development
Stage 3: Fermentation Process Development (3–4 weeks)
• Scale-up from 5L to 500L fermenters
• Real-time DO/pH monitoring for dynamic parameter control
Stage 4: Downstream Purification (2–3 weeks)
• Multi-mode chromatography (affinity/ion exchange/hydrophobic)
• Ultrafiltration for precise final concentration controlS
tage 5: Quality Verification & Delivery
• Basic Testing: SDS-PAGE, HPLC, ELISA
• Advanced Testing: Glycosylation site mapping, bioactivity assays
III. Applications: Empowering Biopharma and Industrial Manufacturing
Full-spectrum solutions from R&D to industrialization:
1. Antibody & Fusion Protein Production
● Monoclonal antibody yields up to 5 g/L, glycoform homogeneity >85%
● Case: PD-1 mAb pilot production saved 60% costs vs. CHO systems
2. Vaccine Antigen Development
● Virus-like particle (VLP) assembly efficiency >80%
● Case: HPV vaccine candidate antigen developed in 6 months
3. Industrial Enzyme Production
● Lipase/amylase fermentation potency increased 3–5x
● Case: Detergent enzyme production cost reduced to $5/g
4. Research Reagent Development
● His-tag, FLAG-tag, and other labeling systems available
● Lyophilized formulations with >24-month ambient stability
5. Diagnostic Antigen Preparation
● Multi-epitope fusion antigen success rate >95%
● Case: HIV antigen test kit sensitivity reached 99.8%
IV. Success Stories: Globally Recognized Milestones
Pharmaceutical Collaboration
• Challenge: High-density fermentation of insulin analogs failed in traditional systems
• Solution: Pichia pastoris secretory expression system
• Result: Fermentation density of 180 g/L (DCW), protein purity >99%
Academic Partnership
• Requirement: Membrane protein with complex glycosylation for structural biology
• Achievement: 2.8Å resolution cryo-EM structure published in Nature Communications
V. Quality System: Full-Cycle Control from Strain to Product
Three-tier quality assurance ensures reliability:
Strain Management
● Master Cell Bank (MCB) validated via whole-genome sequencing
● Monthly phenotypic stability testing
Process Control
● Critical parameter monitoring: Specific growth rate 0.15–0.25 h⁻¹
● Fermentation broth samples archived per batch (storage ≥3 years)
Delivery Standards
● Basic Grade: Purity >90% (SDS-PAGE), concentration error <±5%
● GMP Grade: ICH Q5A compliance with full batch documentation
Customer Benefits
● Technology Transfer: Strain preservation & process validation
● Post-Sales Support: 6 months of technical consultation
Why Choose Ipodix?
● 500+ yeast expression projects completed
● Clients across 20 countries
● 40% faster delivery than industry standards
● Expert team with 10+ years of average experience
Act Now!
● Submit your protein sequence for a feasibility report within 48 hours.
● Free strain screening service
● Priority production capacity allocation
