Analytical Data
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基因名
CCKAR
- Application
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别名
CCKAR；CCKRA；Cholecystokinin receptor type A
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P32238
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表达区间
1-428aa
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氨基酸序列
MDVVDSLLVNGSNITPPCELGLENETLFCLDQPRPSKEWQPAVQILLYSLIFLLSVLGNTLVITVLIRNKRMRTVTNIFLLSLAVSDLMLCLFCMPFNLIPNLLKDFIFGSAVCKTTTYFMGTSVSVSTFNLVAISLERYGAICKPLQSRVWQTKSHALKVIAATWCLSFTIMTPYPIYSNLVPFTKNNNQTANMCRFLLPNDVMQQSWHTFLLLILFLIPGIVMMVAYGLISLELYQGIKFEASQKKSAKERKPSTTSSGKYEDSDGCYLQKTRPPRKLELRQLSTGSSSRANRIRSNSSAANLMAKKRVIRMLIVIVVLFFLCWMPIFSANAWRAYDTASAERRLSGTPISFILLLSYTSSCVNPIIYCFMNKRFRLGFMATFPCCPNPGPPGARGEVGEEEEGGTTGASLSRFSYSHMSASVPPQ
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分子量
47.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
CCKAR (cholecystokinin A receptor) is a G protein-coupled receptor that mediates the physiological effects of cholecystokinin (CCK), a hormone involved in digestion and appetite regulation. Research on CCKAR has gained significant attention due to its potential implications in metabolic disorders, obesity, and gastrointestinal diseases. CCKAR plays a crucial role in pancreatic enzyme secretion, gallbladder contraction, and the modulation of satiety signaling in the brain. Understanding the structure and function of CCKAR is essential for developing selective agonists or antagonists that could serve as therapeutic agents for conditions like obesity and pancreatitis. Additionally, the exploration of CCKAR's signaling pathways and its interactions with other receptors could reveal novel insights into its role in neuroendocrine regulation and its broader impact on human health. The recombinant expression of CCKAR in various systems provides valuable tools for biochemical characterization and drug discovery, enhancing our understanding of its physiological functions and offering new avenues for treatment strategies targeting metabolic and gastrointestinal diseases. Through structural biology techniques, researchers aim to elucidate the receptor's conformational changes and ligand-binding mechanisms, paving the way for innovative therapeutic developments.
