Analytical Data
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Gene name
WWP1
- Application
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Alternative Names
WWP1; NEDD4-like E3 ubiquitin-Protein ligase WWP1; EC 2.3.2.26; Atrophin-1-interacting Protein 5; AIP5; HECT-type E3 ubiquitin transferase WWP1; TGIF-interacting ubiquitin ligase 1; Tiul1; WW domain-containing Protein 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H0M0
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Expression Region
1-251 aa
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AA Sequence
MSAYGMPMYKSGDLVFAKLKGYAHWPARIEHMTQPNRYQVFFFGTHETAFLSPKRLFPYKECKEKFGKPNKRRGFSAGLWEIENNPTVQASDCPLASEKGSGDGPWPEPEAAEGDEDKPTHAGGGGDELGKPDDDKPTEEEKGPLKRSAGDPPEDAPKRPKEAAPDQEEEAEAERAAEAERAAAAAAATAVDEESPFLVAVENGSAPSEPGLVCEPPQPEEEELREEEVADEEASQEWHAEAPGGGDRDSL
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Molecular Weight
53.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
WWP1, or WW Domain Containing E3 Ubiquitin Protein Ligase 1, plays a crucial role in cellular processes by regulating protein degradation and signaling pathways through the ubiquitin-proteasome system. It is involved in various biological functions, including cell cycle regulation, apoptosis, and DNA damage response. Dysregulation of WWP1 has been linked to several diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. Recent studies have focused on the structural and functional characterization of WWP1, revealing its interactions with specific substrates and co-factors. Researchers are particularly interested in understanding the mechanisms by which WWP1 modulates various signaling pathways, such as the TGF-β and Wnt pathways. This research not only enhances our understanding of WWP1's role in normal physiology but also its implications in disease states. The development of WWP1 recombinant proteins has facilitated these investigations, allowing scientists to perform detailed biochemical studies, explore its enzymatic activity, and identify potential inhibitors. Overall, the study of WWP1 and its recombinant forms holds promise for advancing therapeutic strategies against diseases where its regulation is disrupted.
