Analytical Data
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基因名
WWP1
- Application
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别名
WWP1; NEDD4-like E3 ubiquitin-Protein ligase WWP1; EC 2.3.2.26; Atrophin-1-interacting Protein 5; AIP5; HECT-type E3 ubiquitin transferase WWP1; TGIF-interacting ubiquitin ligase 1; Tiul1; WW domain-containing Protein 1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H0M0
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表达区间
1-251 aa
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氨基酸序列
MSAYGMPMYKSGDLVFAKLKGYAHWPARIEHMTQPNRYQVFFFGTHETAFLSPKRLFPYKECKEKFGKPNKRRGFSAGLWEIENNPTVQASDCPLASEKGSGDGPWPEPEAAEGDEDKPTHAGGGGDELGKPDDDKPTEEEKGPLKRSAGDPPEDAPKRPKEAAPDQEEEAEAERAAEAERAAAAAAATAVDEESPFLVAVENGSAPSEPGLVCEPPQPEEEELREEEVADEEASQEWHAEAPGGGDRDSL
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分子量
53.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WWP1, or WW Domain Containing E3 Ubiquitin Protein Ligase 1, plays a crucial role in cellular processes by regulating protein degradation and signaling pathways through the ubiquitin-proteasome system. It is involved in various biological functions, including cell cycle regulation, apoptosis, and DNA damage response. Dysregulation of WWP1 has been linked to several diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. Recent studies have focused on the structural and functional characterization of WWP1, revealing its interactions with specific substrates and co-factors. Researchers are particularly interested in understanding the mechanisms by which WWP1 modulates various signaling pathways, such as the TGF-β and Wnt pathways. This research not only enhances our understanding of WWP1's role in normal physiology but also its implications in disease states. The development of WWP1 recombinant proteins has facilitated these investigations, allowing scientists to perform detailed biochemical studies, explore its enzymatic activity, and identify potential inhibitors. Overall, the study of WWP1 and its recombinant forms holds promise for advancing therapeutic strategies against diseases where its regulation is disrupted.
