Analytical Data
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Gene name
ZDHHC23
- Application
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Alternative Names
ZDHHC23; Palmitoyltransferase ZDHHC23; Zinc finger DHHC domain-containing protein 23; DHHC-23; zDHHC23
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IYP9
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Expression Region
1-409 aa
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AA Sequence
MTQKGSMKPVKKKKTEEPELEPLCCCEYIDRNGEKNHVATCLCDCQDLDEGCDRWITCKSLQPETCERIMDTISDRLRIPWLRGAKKVNISIIPPLVLLPVFLHVASWHFLLGVVVLTSLPVLALWYYYLTHRRKEQTLFFLSLGLFSLGYMYYVFLQEVVPKGRVGPVQLAVLTCGLFLILLALHRAKKNPGYLSNPASGDRSLSSSQLECLSRKGQEKTKGFPGADMSGSLNNRTTKDDPKGSSKMPAGSPTKAKEDWCAKCQLVRPARAWHCRICGICVRRMDHHCVWINSCVGESNHQAFILALLIFLLTSVYGITLTLDTICRDRSVFTALFYCPGVYANYSSALSFTCVWYSVIITAGMAYIFLIQLINISYNVTEREVQQALRQKTGRRLLCGLIVDTGLLG
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Molecular Weight
46.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
ZDHHC23 (Zinc Finger DHHC-Type Palmitoyltransferase 23) is a member of the DHHC protein family, known for its role in the post-translational modification of proteins through palmitoylation. This modification involves the addition of palmitic acid, a saturated fatty acid, to cysteine residues in target proteins, which can significantly impact protein function, localization, and interactions. Research has shown that ZDHHC23 is implicated in various cellular processes, including neurite development and synaptic function, suggesting its importance in neurobiology. Mutations in the ZDHHC23 gene have been linked to neurodevelopmental disorders, highlighting its potential role in disease mechanisms. Furthermore, palmitoylation mediated by ZDHHC proteins plays crucial roles in membrane dynamics, signaling pathways, and the regulation of protein stability. The study of ZDHHC23's structure and function is not only essential for understanding the molecular basis of its involvement in cellular processes but also offers insight into the therapeutic potential of targeting palmitoylation pathways in diseases. Investigating the recombinant expression and biochemical characterization of ZDHHC23 can provide crucial data on its enzymatic activity, substrate specificity, and regulatory mechanisms, leading to a deeper understanding of its physiological roles and potential as a therapeutic target. As the field of lipid modifications garners increasing interest, ZDHHC23 represents a key player in the intricate network of cellular signaling and function, positioning it as a significant focus for future research endeavors.
