Analytical Data
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Gene name
WHSC1
- Application
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Alternative Names
FLJ23286; IL5 promoter REII region binding Protein; KIAA1090; MGC176638; MMSET; MMSET type II; Multiple myeloma SET domain containing Protein type III; Multiple myeloma SET domain Protein; Multiple myeloma SET domain-containing Protein; NSD 2; NSD2; NSD2_HUMAN; Nuclear receptor binding SET domain Protein 2; Nuclear SET domain-containing Protein 2; Probable histone-lysine N-methyltransferase NSD2; Protein trithorax-5; REIIBP; Trithorax/ash1 related Protein 5; TRX5; TRX5 Protein ; WHS; Whsc1; Wolf Hirschhorn syndrome candidate 1; Wolf Hirschhorn syndrome candidate 1 Protein; Wolf-Hirschhorn syndrome candidate 1 Protein
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O96028
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Expression Region
1-647 aa
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AA Sequence
MEFSIKQSPLSVQSVVKCIKMKQAPEILGSANGKTPSCEVNRECSVFLSKAQLSSSLQEGVMQKFNGHDALPFIPADKLKDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNGSPEIKLKITKTYMNGKPLFESSICGDSAADVSQSEENGQKPENKARRNRKRSIKYDSLLEQGLVEAALVSKISSPSDKKIPAKKESCPNTGRDKDHLLKYNVGDLVWSKVSGYPWWPCMVSADPLLHSYTKLKGQKKSARQYHVQFFGDAPERAWIFEKSLVAFEGEGQFEKLCQESAKQAPTKAEKIKLLKPISGKLRAQWEMGIVQAEEAASMSVEERKAKFTFLYVGDQLHLNPQVAKEAGIAAESLGEMAESSGVSEEAAENPKSVREECIPMKRRRRAKLCSSAETLESHPDIGKSTPQKTAEADPRRGVGSPPGRKKTTVSMPRSRKGDAASQFLVFCQKHRDEVVAEHPDASGEEIEELLRSQWSLLSEKQRARYNTKFALVAPVQAEEDSGNVNGKKRNHTKRIQDPTEDAEAEDTPRKRLRTDKHSLRKRDTITDKTARTSSYKAMEAASSLKSQAATKNLSDACKPLKKRNRASTAASSALGFSKSSSPSASLTENELLWEPTPVKLDLNPAALYCT
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Molecular Weight
97.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
WHSC1, or Wolf-Hirschhorn syndrome candidate 1, is a gene located on chromosome 4 that plays a pivotal role in various cellular processes, including transcription regulation, chromatin remodeling, and DNA damage repair. The WHSC1 protein is a histone methyltransferase, specifically involved in the trimethylation of lysine 36 on histone H3 (H3K36me3), a modification associated with active transcription and DNA repair mechanisms. Mutations in the WHSC1 gene are linked to Wolf-Hirschhorn syndrome, a rare genetic disorder characterized by developmental delays, facial dysmorphism, and intellectual disability. The study of WHSC1 recombinant protein is crucial for understanding its biological functions and implications in disease pathology. By expressing and purifying WHSC1 in a recombinant system, researchers can investigate its enzymatic activity, structural characteristics, and interactions with other cellular proteins. This knowledge can shed light on the dysregulation of epigenetic processes in various cancers and developmental disorders, where WHSC1 activity may be altered. Furthermore, elucidating the molecular mechanisms of WHSC1 could offer insights into potential therapeutic strategies for conditions associated with its dysfunction. Overall, the research on WHSC1 recombinant protein not only enhances our understanding of fundamental biological processes but also opens avenues for clinical applications in the management of genetic disorders and cancer treatments.
