Analytical Data
-
Gene name
SEMA3D
- Application
-
Alternative Names
Coll 2; Coll2 ; Collapsin 2; SEM3D_HUMAN; Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3D; Sema Z2; Sema3d; Semaphorin-3D; Semaphorin3D
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O95025
-
Expression Region
37-777 aa
-
AA Sequence
TLKQ NIPRLKLTYK DLLLSNSCIP FLGSSEGLDF QTLLLDEERG RLLLGAKDHI FLLSLVDLNK NFKKIYWPAA KERVELCKLA GKDANTECAN FIRVLQPYNK THIYVCGTGA FHPICGYIDL GVYKEDIIFK LDTHNLESGR LKCPFDPQQP FASVMTDEYL YSGTASDFLG KDTAFTRSLG PTHDHHYIRT DISEHYWLNG AKFIGTFFIP DTYNPDDDKI YFFFRESSQE GSTSDKTILS RVGRVCKNDV GGQRSLINKW TTFLKARLIC SIPGSDGADT YFDELQDIYL LPTRDERNPV VYGVFTTTSS IFKGSAVCVY SMADIRAVFN GPYAHKESAD HRWVQYDGRI PYPRPGTCPS KTYDPLIKST RDFPDDVISF IKRHSVMYKS VYPVAGGPTF KRINVDYRLT QIVVDHVIAE DGQYDVMFLG TDIGTVLKVV SISKEKWNME EVVLEELQIF KHSSIILNME LSLKQQQLYI GSRDGLVQLS LHRCDTYGKA CADCCLARDP YCAWDGNACS RYAPTSKRRA RRQDVKYGDP ITQCWDIEDS ISHETADEKV IFGIEFNSTF LECIPKSQQA TIKWYIQRSG DEHREELKPD ERIIKTEYGL LIRSLQKKDS GMYYCKAQEH TFIHTIVKLT LNVIENEQME NTQRAEHEEG KVKDLLAESR LRYKDYIQIL SSPNFSLDQY CEQMWHREKR RQRNKGGPKW KHMQEMKKKR NRRHHRDLDE LPRAVAT
-
Molecular Weight
89.6 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SEMA3D, a member of the Semaphorin family, is a class of proteins originally identified for their roles in guiding neuronal development and axon pathfinding. Recent research has expanded the interest in SEMA3D due to its potential involvement in various physiological and pathological processes, including immune responses, cancer progression, and vascular development. It functions by interacting with specific receptors, primarily neuropilins and plexins, influencing cell migration and signaling pathways. Understanding the structure and function of SEMA3D through recombinant protein studies enables researchers to explore its mechanisms in detail, potentially revealing therapeutic targets for conditions such as tumors and neurodegenerative diseases. The development of SEMA3D recombinant proteins has facilitated investigations into its biological activities and interactions, paving the way for novel strategies in regenerative medicine and targeted therapies. Researchers are particularly focused on elucidating the molecular pathways modulated by SEMA3D, contributing to a greater understanding of its role in cellular communication and tissue homeostasis. Overall, the study of SEMA3D and its recombinant forms holds significant promise for advancing our knowledge of cell biology and developing innovative therapeutic interventions.
