Analytical Data
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Gene name
CLCNKB
- Application
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Alternative Names
Bartter syndrome type 3; Chloride channel Kb; Chloride channel kidney B; Chloride channel protein ClC-Kb; Chloride channel voltage sensitive Kb; ClC K2; ClC-K2; ClCK2; CLCKB; CLCKB_HUMAN; CLCNKB; hClC Kb
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51801
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Expression Region
1-462aa
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AA Sequence
MEEFVGLREGSSGNPVTLQELWGPCPRIRRGIRGGLEWLKQKLFRLGEDWYFLMTLGVLMALVSCAMDLAVESVVRAHQWLYREIGDSHLLRYLSWTVYPVALVSFSSGFSQSITPSSGGSGIPEVKTMLAGVVLEDYLDIKNFGAKVVGLSCTLACGSTLFLGKVGPFVHLSVMMAAYLGRVRTTTIGEPENKSKPVYSALATLVLASITYPPSAGRFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFVYGAAIGRLFGETLSFIFPEGIVAGGITNPIMPGGYALAGAAAFSGAVTHTISTALLAFEVTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTVIVKKLPYLPRILGRNIGSHRVRVEHFMNHSITTLAITLMNDEIGVHCHQGQAQMASGVVWFPVRGS
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Molecular Weight
77 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CLCNKB, a member of the ClC family of chloride channels, plays a crucial role in maintaining ion homeostasis and cell volume regulation. Mutations in the CLCNKB gene are associated with Bartter syndrome type III, a genetic disorder characterized by hypokalemic metabolic alkalosis and impaired renal chloride reabsorption. Despite its importance in kidney function and electrolyte balance, the structural and functional mechanisms underlying CLCNKB's activities remain inadequately understood. Recent advancements in protein expression and purification techniques have enabled researchers to recombine CLCNKB into a functional form suitable for detailed biophysical and biochemical analyses. These studies aim to elucidate its ion transport mechanisms, interaction with regulatory proteins, and response to pharmacological agents. Understanding CLCNKB's properties may pave the way for novel therapeutic strategies targeting electrolyte imbalances and related disorders, offering insights into potential treatment options for Bartter syndrome and other renal dysfunctions. The ongoing research into the recombinant expression of CLCNKB emphasizes the channel's significance not only in basic science but also in its potential clinical implications, making it a compelling focus for further investigation in both pharmacology and molecular biology.
