Analytical Data
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Gene name
LEPR
- Application
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Alternative Names
LEPR；DB；OBR；Leptin receptor
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48357
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Expression Region
22-839aa
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AA Sequence
FNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPK FNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQ QIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLED SPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVI FQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQV KYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSD WSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMN LAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHR YAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSL YCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGS LDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQ IRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGL GYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMK NDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINS IGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMY FIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGV GKPKIINSFTQDDIEKHQSD
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Molecular Weight
94 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Leptin is a critical hormone involved in regulating energy balance and body weight, primarily produced by adipocytes. Its mechanism of action is mediated by the leptin receptor (LEPR), which is a member of the class I cytokine receptor family. Variations in LEPR signaling can lead to obesity and metabolic disorders, as impaired leptin function diminishes the body's ability to regulate hunger and energy expenditure. Recombinant LEPR protein studies have emerged as a valuable tool to elucidate the receptor's structure, function, and its complex signaling pathways. By employing recombinant DNA technology, researchers can produce large quantities of LEPR, allowing for detailed biochemical and biophysical characterization. These studies aim to identify the receptor’s interactions with various ligands and downstream signaling molecules, which are crucial for understanding how LEPR dysfunction contributes to obesity and its related conditions. The generation of LEPR mutants also facilitates investigations into the effects of specific genetic variations on receptor functionality. Overall, research involving LEPR recombinant proteins holds significant promise for developing therapeutic strategies targeting leptin signaling to combat obesity and metabolic diseases.
