Analytical Data
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Gene name
WDR5
- Application
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Alternative Names
WDR5；BIG3；WD repeat-containing Protein 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P61964
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Expression Region
2-334aa
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AA Sequence
ATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC
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Molecular Weight
52.5kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
WDR5 (WD Repeat Containing Protein 5) is a crucial component of the MLL (Mixed-Lineage Leukemia) complex, which plays a significant role in gene regulation and chromatin modification. It is known to associate with histone methyltransferases, facilitating the trimethylation of histone H3 at lysine 4 (H3K4me3), a key epigenetic mark associated with active transcription. Due to its pivotal role in developmental processes and cellular differentiation, dysregulation of WDR5 has been implicated in various diseases, particularly in hematological malignancies, including acute leukemia. Researchers have shown that WDR5 interacts with several transcription factors and co-factors, influencing multiple signaling pathways. Given its central role in oncogenic processes, WDR5 has emerged as a promising target for therapeutic intervention. Recent studies involving the development of recombinant WDR5 proteins have provided insights into its structural properties, interaction dynamics, and functional implications in transcriptional regulation. The characterization of WDR5 not only enhances our understanding of its role in normal physiology but also offers potential avenues for innovative cancer therapeutics that could disrupt aberrant gene expression in malignancies where WDR5 is overexpressed or mutated. Overall, the study of WDR5 recombinant proteins is an important area of research that bridges fundamental biology with clinical applications, highlighting the need for further exploration of its mechanisms of action and potential as a drug target.
