Analytical Data
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Gene name
ATP6V1G2
- Application
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Alternative Names
ATP6G; ATP6G2; ATP6V1G2; ATPase H+ transporting lysosomal (vacuolar proton pump) subunit G
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95670
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Expression Region
1-226aa
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AA Sequence
MALSDVDVKKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKILMSTMRNQARLKVLRARNDLISDLLSEAKLRLSRIVEDPEVYQGLLDKLVLQGLLRLLEPVMIVRCRPQDLLLVEAAVQKAIPEYMTISQKHVEVQIDKEAYLAVNAAGGVEVYSGNQRIKVSNTLESRLDLSAKQKMPEIRMALFGANTNRKFFI
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Molecular Weight
52.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP6V1G2, a gene encoding a subunit of the vacuolar ATPase (V-ATPase), plays a crucial role in the acidic environment of various cellular compartments, influencing processes such as protein sorting, nutrient uptake, and cellular signaling. Research into ATP6V1G2 has gained momentum due to its potential implications in various diseases, including cancer and neurodegenerative disorders. Abnormal expression of ATP6V1G2 has been associated with tumor progression and metastasis, highlighting its significance in the field of cancer biology. Moreover, studies suggest that ATP6V1G2 may be involved in the regulation of intracellular pH and ion homeostasis, which are essential for maintaining cellular functions. Investigating the structure and function of the recombinant ATP6V1G2 protein can provide insights into its specific roles in cellular mechanisms and disease pathogenesis. Producing a recombinant form of this protein allows researchers to examine its properties, interactions, and regulatory functions in vitro, thus offering a deeper understanding of its involvement in key biological processes. This knowledge could pave the way for the development of targeted therapies that manipulate V-ATPase activity, potentially leading to novel treatment strategies for diseases characterized by dysregulated cellular environments. As a result, the study of ATP6V1G2 is not only vital for basic research but also holds promise for translational applications in medicine, making it an important focus within the fields of molecular biology and biochemistry.
