Analytical Data
-
Gene name
PDE6H
- Application
-
Alternative Names
5''-cyclic phosphodiesterase subunit gamma; CNCG_HUMAN; GMP-PDE gamma; Pde6h; Retinal cone rhodopsin-sensitive cGMP 3''
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q13956
-
Expression Region
1-83 aa
-
AA Sequence
MSDNTTLPAP ASNQGPTTPR KGPPKFKQRQ TRQFKSKPPK KGVKGFGDDI PGMEGLGTDI TVICPWEAFS HLELHELAQF GII
-
Molecular Weight
9.0 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDE6H, or phosphodiesterase 6H, is a critical enzyme involved in the phototransduction pathway in retinal photoreceptor cells, primarily in rods. This enzyme plays a significant role in the hydrolysis of cyclic GMP (cGMP), thereby regulating the levels of this important second messenger during light signaling. Dysfunction in PDE6H has been linked to various retinal diseases, including retinitis pigmentosa and congenital stationary night blindness, emphasizing the importance of understanding its biological role and mechanisms. Recent studies have focused on the recombination and characterization of PDE6H proteins to elucidate their structure-function relationships. Such research can provide insights into the enzyme's catalytic action, inhibition mechanisms, and potential therapeutic targets in retinal disorders. The production of recombinant PDE6H proteins enables detailed biochemical assays, structural biology studies, and drug screening processes, enhancing our understanding of visual signal transduction. Consequently, PDE6H has gained attention for its potential role in developing targeted therapies for retinal diseases, driving an interest in its recombinant protein studies in the field of molecular biology and genetics.
