Analytical Data
-
Gene name
H1
- Application
-
Alternative Names
H1;H1FX;Histone H1.10
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q8IZA3
-
Expression Region
1-346aa
-
AA Sequence
MAPGSVTSDI SPSSTSTAGS SRSPESEKPG PSHGGVPPGG PSHSSLPVGR RHPPVLRMVL EALQAGEQRR GTSVAAIKLY ILHKYPTVDV LRFKYLLKQA LATGMRRGLL ARPLNSKARG ATGSFKLVPK HKKKIQPRKM APATAPRRAG EAKGKGPKKP SEAKEDPPNV GKVKKAAKRP AKVQKPPPKP GAATEKARKQ GGAAKDTRAQ SGEARKVPPK PDKAMRAPSS AGGLSRKAKA KGSRSSQGDA EAYRKTKAES KSSKPTASKV KNGAASPTKK KVVAKAKAPK AGQGPNTKAA APAKGSGSKV VPAHLSRKTE APKGPRKAGL PIKASSSKVS SQRAEA
-
Molecular Weight
35.8 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
H1 histone proteins play a crucial role in the packaging of DNA within the nucleus, contributing to the formation of higher-order chromatin structures. As the major component of the nucleosome, H1 functions in stabilizing the nucleosome and regulating DNA accessibility, which is essential for processes such as transcription, replication, and repair. Research into H1 proteins has gained significant attention due to their involvement in various cellular processes and their potential implications in diseases, including cancer. Abnormal expression or mutation of H1 histones can lead to altered chromatin dynamics and, consequently, dysfunctional gene regulation. Studies have focused on deciphering the specific roles of different H1 variants, understanding their interactions with other chromatin components, and exploring their post-translational modifications. Furthermore, advances in molecular biology techniques have enabled researchers to investigate H1’s impact on epigenetic regulation and cellular differentiation, making it a vital target for therapeutic interventions. Overall, the exploration of H1 histone proteins not only enhances our fundamental understanding of chromatin biology but also opens avenues for novel diagnostic and treatment strategies in diseases associated with epigenetic abnormalities.











