Analytical Data
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Gene name
OPA1
- Application
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Alternative Names
OPA1；KIAA0567；Dynamin-like GTPase OPA1. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60313
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Expression Region
1-960aa
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AA Sequence
MWRLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTLKL QRPQLRTSFQQFSSLTNLPLRKLKFSPIKYGYQPRRNFWPARLATRLLKL RYLILGSAVGGGYTAKKTFDQWKDMIPDLSEYKWIVPDIVWEIDEYIDFE KIRKALPSSEDLVKLAPDFDKIVESLSLLKDFFTSGSPEETAFRATDRGS ESDKHFRKVSDKEKIDQLQEELLHTQLKYQRILERLEKENKELRKLVLQK DDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQSAG KTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDL TKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMVLVDLP GVINTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTD LVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKLFPMKALGYFA VVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSD CFWKMVRESVEQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNE ILDEVISLSQVTPKHWEEILQQSLWERVSTHVIENIYLPAAQTMNSGTFN TTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKL KEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEE ALQARLKDTENAIENMVGPDWKKRWLYWKNRTQEQCVHNETKNELEKMLK CNEEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTAL NHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQRMLAITANTLRQQLTN TEVRRLEKNVKEVLEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLD AFIEALHQEK
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Molecular Weight
138 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
OPA1 (Optic Atrophy 1) is a dynamin-related GTPase that plays a crucial role in mitochondrial dynamics, primarily in the regulation of mitochondrial fusion and maintenance of cristae structure. Mutations in the OPA1 gene are associated with dominant optic atrophy, a hereditary condition that leads to vision loss due to the degeneration of retinal ganglion cells. Research on OPA1 recombinant proteins has gained prominence as scientists seek to understand the molecular mechanisms underlying mitochondrial dysfunction in various diseases, including neurodegenerative disorders and metabolic syndromes. The study of OPA1 encompasses its interactions with other mitochondrial proteins, its role in apoptosis, and how it influences the overall mitochondrial morphology and bioenergetics. Additionally, exploring the potential for developing therapeutic interventions based on OPA1 modulation is of significant interest, as enhancing mitochondrial function may offer protective effects against cellular stress and degeneration. In this context, producing and characterizing OPA1 recombinant proteins can aid in elucidating its functional properties and interactions, providing valuable insights into its contribution to mitochondrial health and the pathophysiology of associated disorders.
