Analytical Data
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Gene name
tetX3
- Application
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Species
Pseudomonas aeruginosa
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7X2A0
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Expression Region
1-378aa
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Molecular Weight
46.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The tetX3 gene encodes a class of tetracycline resistance enzymes, specifically belonging to the tetX family, which are known to catalyze the modification of tetracycline antibiotics, thereby rendering them ineffective. The prevalence of tetracycline resistance is a pressing public health concern, especially with the rise of multidrug-resistant bacterial strains. Researchers are increasingly focused on the mechanisms of antibiotic resistance to develop effective strategies to combat resistant infections. tetX3 has garnered attention due to its unique enzymatic properties and its ability to confer resistance to a wider range of tetracycline derivatives compared to its predecessors. Investigating the recombinant protein of tetX3 allows for a deeper understanding of its structure-function relationships, substrate specificity, and the biochemical pathways involved in antibiotic resistance. Furthermore, through recombinant expression systems, scientists can produce tetX3 in sufficient quantities for detailed studies, including kinetics, crystallography, and inhibitor screening. The research aims not only to elucidate the mechanisms of resistance but also to inform the development of novel therapeutic agents or inhibitors that can target mechanisms of antibiotic resistance. Given the global rise in antibiotic-resistant infections, understanding tetX3 and similar genes is crucial for shaping future antibiotic stewardship and developing new strategies to enhance the efficacy of existing antibiotics.
