Analytical Data
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Gene name
PRODH
- Application
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Alternative Names
PRODH；PIG6；POX2；PRODH2；Proline dehydrogenase 1. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43272
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Expression Region
1-600aa
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AA Sequence
MALRRALPALRPCIPRFVPLSTAPASREQPAAGPAAVPGGGSATAVRPPVPAVDFGNAQEAYRSRRTWELARSLLVLRLCAWPALLARHEQLLYVSRKLLGQRLFNKLMKMTFYGHFVAGEDQESIQPLLRHYRAFGVSAILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHWAFGDRRNGVISARTYFYANEAKCDSHMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFSEVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAYDNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAKAKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPYLSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA
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Molecular Weight
68 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRODH (proline dehydrogenase) is an enzyme that plays a crucial role in the catabolism of proline, an amino acid that serves as a significant energy source and is involved in various metabolic pathways. The enzymatic activity of PRODH is important not just for proline metabolism but also for regulation of oxidative stress and cellular signaling. Research has shown that altered PRODH expression is associated with several pathological conditions, including hyperprolinemia, neurodegenerative diseases, and certain cancers, indicating its potential role as a therapeutic target. The development of recombinant PRODH proteins allows for in-depth studies of its structure-function relationships, kinetic properties, and interactions with other metabolic pathways. By producing these proteins through recombinant DNA technology, researchers can better understand the enzyme’s mechanism, investigate how mutations may lead to disease, and explore its applications in metabolic engineering and biotechnology. Understanding the functional differences between isoforms of PRODH, for instance, can provide insights into enzyme regulation and the potential development of selective inhibitors or activators, paving the way for novel therapeutic strategies aimed at diseases linked to dysregulated proline metabolism. Thus, PRODH not only stands as a vital enzyme in metabolic processes but also as a promising target for biomedical research and therapeutic interventions.
