Analytical Data
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基因名
PRODH
- Application
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别名
PRODH；PIG6；POX2；PRODH2；Proline dehydrogenase 1. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43272
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表达区间
1-600aa
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氨基酸序列
MALRRALPALRPCIPRFVPLSTAPASREQPAAGPAAVPGGGSATAVRPPVPAVDFGNAQEAYRSRRTWELARSLLVLRLCAWPALLARHEQLLYVSRKLLGQRLFNKLMKMTFYGHFVAGEDQESIQPLLRHYRAFGVSAILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHWAFGDRRNGVISARTYFYANEAKCDSHMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFSEVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAYDNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAKAKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPYLSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA
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分子量
68 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PRODH (proline dehydrogenase) is an enzyme that plays a crucial role in the catabolism of proline, an amino acid that serves as a significant energy source and is involved in various metabolic pathways. The enzymatic activity of PRODH is important not just for proline metabolism but also for regulation of oxidative stress and cellular signaling. Research has shown that altered PRODH expression is associated with several pathological conditions, including hyperprolinemia, neurodegenerative diseases, and certain cancers, indicating its potential role as a therapeutic target. The development of recombinant PRODH proteins allows for in-depth studies of its structure-function relationships, kinetic properties, and interactions with other metabolic pathways. By producing these proteins through recombinant DNA technology, researchers can better understand the enzyme’s mechanism, investigate how mutations may lead to disease, and explore its applications in metabolic engineering and biotechnology. Understanding the functional differences between isoforms of PRODH, for instance, can provide insights into enzyme regulation and the potential development of selective inhibitors or activators, paving the way for novel therapeutic strategies aimed at diseases linked to dysregulated proline metabolism. Thus, PRODH not only stands as a vital enzyme in metabolic processes but also as a promising target for biomedical research and therapeutic interventions.
