Analytical Data
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Gene name
SQSTM1
- Application
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Alternative Names
Sqstm1;A170;STAP;Sequestosome-1
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Species
Rat
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Source
E. coli
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Tag
N-terminal His Tag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O08623
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Expression Region
2-439aa
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AA Sequence
ASLTVKAYL LGKEEAAREIRRFSFCFSPE PEAEAAAGPG PCERLLSRVAVLFPALRPGG FQAHYRDEDGDLVAFSSDEE LTMAMSYVKD DIFRIYIKEKKECRREHRPP CAOEARSMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSV CEGKGLHREH SKLIFPNPFG HLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRA GDGRPCPTAE SASAPSEDPN VNFLKNVGESVAAALSPLGIEVDIDVEHGG KRSRLTPTSA ESSSTGTEDKSGTOPSSCSS EVSKPDGAGEGPAQSLTEQM KKIALESVGQPEELMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPES EGPSSLDPSQ EGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGG WLTRLLQTKN YDIGAALDTI QYSKHPPPL
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Molecular Weight
51.3kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, 5% Trehalose
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
SQSTM1, also known as Sequestosome 1, is a multifunctional protein that plays a crucial role in cellular processes such as autophagy, the regulation of inflammation, and the maintenance of protein homeostasis. It serves as a scaffold for various signaling pathways, linking the ubiquitin-proteasome system with autophagy. The study of SQSTM1 has gained significant attention due to its association with several neurodegenerative diseases, including Amyotrophic Lateral Sclerosis (ALS) and frontotemporal dementia (FTD), where mutations in the SQSTM1 gene have been linked to disease onset and progression. As a key player in the cellular degradation pathways, SQSTM1's function is tightly regulated, and its dysregulation is implicated in the accumulation of misfolded proteins, thus contributing to neuronal toxicity. Research into SQSTM1 recombinant proteins aims to elucidate its structural and functional properties, providing insights into its role in disease mechanisms and potential therapeutic targets. By producing and analyzing SQSTM1 recombinants, scientists hope to better understand the protein's interactions and regulatory mechanisms, ultimately leading to improved strategies for modulating its function in the context of neurodegenerative diseases. This area of study not only advances the basic understanding of protein dynamics within cells but also opens new avenues for developing innovative treatments for related disorders.
