Analytical Data
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Gene name
PINK1
- Application
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Alternative Names
PINK1;Serine/threonine-Protein kinase PINK1. mitochondrial
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Species
Human
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Source
E. coli
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Tag
N-terminal His-Tag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BXM7
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Expression Region
262~507aa
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AA Sequence
KRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLF LVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNIL VELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARP GPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPES VPPDVRQLVRALLQREASKRPSARVAANVL
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Molecular Weight
30.6kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
PINK1 (PTEN-induced putative kinase 1) is a serine/threonine kinase that plays a crucial role in mitochondrial quality control and the regulation of cell death pathways. Mutations in the PINK1 gene are associated with autosomal recessive forms of Parkinson's disease, highlighting its significance in neurodegenerative disorders. PINK1 functions by sensing mitochondrial dysfunction and initiating protective mechanisms, including the activation of the Parkin protein, which tags damaged mitochondria for degradation through mitophagy. The study of recombinant PINK1 protein has gained momentum due to its potential therapeutic implications and the need for a better understanding of its enzymatic activity and interaction with other mitochondrial proteins. Researchers are focused on overexpressing and purifying PINK1 in various systems to study its structure, function, and biochemical characteristics. This research is essential for elucidating the molecular mechanisms underlying PINK1-related pathologies and may lead to novel strategies for the treatment of Parkinson's disease and other mitochondrial dysfunction-associated conditions. By understanding the biological pathways mediated by PINK1, scientists aim to develop targeted therapies that can enhance mitochondrial function and protect neuronal health.
