Analytical Data
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基因名
vpr
- Application
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别名
vpr;KIAA0800;RIP;VPRBP;DDB1- and CUL4-associated factor 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O12160
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表达区间
1-96aa
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氨基酸序列
MEQAPEDQGPQREPYNEWTLELLEELKREAVRHFPRPWLHGLGQHIYETYGDTWTGVEAIIRILQRLLFVHFRIGCQHSRIGILRQRRARNGASRS
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分子量
18.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Vpr (Viral protein R) is a unique accessory protein encoded by the human immunodeficiency virus type 1 (HIV-1) that plays critical roles in viral replication and pathogenesis. It is known to be involved in various cellular processes, including cell cycle regulation, apoptosis, and immune evasion, which are essential for the efficient replication of the virus. Research on Vpr has gained considerable attention due to its multifunctional nature and its potential as a target for therapeutic interventions against HIV. The protein is capable of interacting with host cellular factors, facilitating viral gene expression, and enhancing viral production. Additionally, Vpr has been implicated in promoting viral infectivity and the establishment of viral reservoirs in the host, complicating eradication efforts. The study of Vpr and its interactions at a molecular level is crucial for understanding the viral lifecycle and the mechanisms it employs to subvert host defenses. Moreover, the rational design of Vpr-based antiviral strategies could offer novel avenues for HIV treatment. Recent advances in recombinant protein technology have enabled researchers to produce Vpr in a laboratory setting, making it easier to investigate its structure-function relationships and to explore its role in HIV pathogenesis further. Overall, the ongoing research into Vpr is not only pertinent for understanding HIV biology but also for developing innovative therapeutic approaches to combat this global health challenge.












