Analytical Data
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Gene name
HDAC9
- Application
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Alternative Names
HDAC9；HDAC7；HDAC7B；HDRP；Histone deacetylase 9
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UKV0
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Expression Region
548-1011aa
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AA Sequence
KEEPVDSDEDAQIQEMESGEQAAFMQQPFLEPTHTRALSVRQAPLAAVGM DGLEKHRLVSRTHSSPAASVLPHPAMDRPLQPGSATGIAYDPLMLKHQCV CGNSTTHPEHAGRIQSIWSRLQETGLLNKCERIQGRKASLEEIQLVHSEH HSLLYGTNPLDGQKLDPRILLGDDSQKFFSSLPCGGLGVDSDTIWNELHS SGAARMAVGCVIELASKVASGELKNGFAVVRPPGHHAEESTAMGFCFFNS VAITAKYLRDQLNISKILIVDLDVHHGNGTQQAFYADPSILYISLHRYDE GNFFPGSGAPNEVGTGLGEGYNINIAWTGGLDPPMGDVEYLEAFRTIVKP VAKEFDPDMVLVSAGFDALEGHTPPLGGYKVTAKCFGHLTKQLMTLADGR VVLALEGGHDLTAICDASEACVNALLGNELEPLAEDILHQSPNMNAVISL QKIIEIQSMSLKFS
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Molecular Weight
77 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Histone deacetylases (HDACs) play a crucial role in the regulation of gene expression through the removal of acetyl groups from lysine residues on histone proteins, influencing chromatin structure and function. HDAC9, a member of the Class II HDAC family, has garnered significant attention due to its involvement in various physiological and pathological processes, including development, inflammation, and cancer. Unlike Class I HDACs, Class II HDACs, including HDAC9, are characterized by their ability to be regulated by cellular signaling pathways and can shuttle between the nucleus and cytoplasm, affecting gene expression in a context-dependent manner. Recent studies have highlighted HDAC9's role beyond histone modification, indicating its involvement in the regulation of non-coding RNAs and interaction with transcription factors that could influence cellular responses. The growing body of evidence suggests that HDAC9 may serve as a potential therapeutic target for diseases where HDAC activity is dysregulated. Recombining HDAC9 protein facilitates the investigation of its structure-function relationship, enzymatic activity, and interaction with other biomolecules, ultimately aiding in the development of HDAC inhibitors as therapeutic agents. Understanding the molecular mechanisms by which HDAC9 operates in different cellular contexts could be instrumental in identifying novel strategies for treatment in cancer and other diseases associated with aberrant gene expression.
