Analytical Data
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Gene name
HDAC4
- Application
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Alternative Names
HDAC4；KIAA0288；Histone deacetylase 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P56524
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Expression Region
101-1084aa
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AA Sequence
SRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQELEKQHREQ KLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCISSDPR YWYGKTQHSSLDQSSPPQSGVSTSYNHPVLGMYDAKDDFPLRKTASEPNL KLRSRLKQKVAERRSSPLLRRKDGPVVTALKKRPLDVTDSACSSAPGSGP SSPNNSSGSVSAENGIAPAVPSIPAETSLAHRLVAREGSAAPLPLYTSPS LPNITLGLPATGPSAGTAGQQDAERLTLPALQQRLSLFPGTHLTPYLSTS PLERDGGAAHSPLLQHMVLLEQPPAQAPLVTGLGALPLHAQSLVGADRVS PSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFLEKHKQQFQQQ QLQMNKIIPKPSEPARQPESHPEETEEELREHQALLDEPYLDRLPGQKEA HAQAGVQVKQEPIESDEEEAEPPREVEPGQRQPSEQELLFRQQALLLEQQ RIHQLRNYQASMEAAGIPVSFGGHRPLSRAQSSPASATFPVSVQEPPTKP RFTTGLVYDTLMLKHQCTCGSSSSHPEHAGRIQSIWSRLQETGLRGKCEC IRGRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSKKLLGSLASVFVRLP CGGVGVDSDTIWNEVHSAGAARLAVGCVVELVFKVATGELKNGFAVVRPP GHHAEESTPMGFCYFNSVAVAAKLLQQRLSVSKILIVDWDVHHGNGTQQA FYSDPSVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDP PMGDAEYLAAFRTVVMPIASEFAPDVVLVSSGFDAVEGHPTPLGGYNLSA RCFGYLTKQLMGLAGGRIVLALEGGHDLTAICDASEACVSALLGNELDPL PEKVLQQRPNANAVRSMEKVMEIHSKYWRCLQRTTSTAGRSLIEAQTCEN EEAETVTAMASLSVGVKPAEKRPDEEPMEEEPPL
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Molecular Weight
155kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Histone deacetylase 4 (HDAC4) is a member of the HDAC family, which plays a crucial role in the regulation of gene expression by removing acetyl groups from histone proteins, thereby leading to chromatin condensation and gene repression. Increased attention has been drawn to HDAC4 due to its involvement in various biological processes, including cell differentiation, apoptosis, and neurodevelopment. Emerging studies have linked HDAC4 to several diseases, particularly cancer and neurodegenerative disorders, where its dysregulation contributes to altered gene expression patterns that promote malignancy and neurotoxicity. The therapeutic potential of targeting HDAC4 is underscored by the development of selective inhibitors that can modulate its activity and restore normal gene expression. Furthermore, research into the structural and functional characteristics of recombinant HDAC4 proteins aids in the understanding of its mechanisms and interactions with other cellular factors. Expression and purification of HDAC4 as a recombinant protein provide valuable tools for high-throughput screening assays, structural biology studies, and evaluating its role in cellular pathways. Thus, the investigation of HDAC4 recombinants not only enhances our understanding of epigenetic regulation but also opens avenues for novel therapeutic strategies aimed at diseases associated with HDAC4 dysregulation.
