Analytical Data
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基因名
HIST2H3A
- Application
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别名
HIST2H3A；HIST2H3A；H3C14；H3F2；Histone H3.2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q71DI3
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表达区间
2-136aa
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氨基酸序列
ARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEASEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HIST2H3A encodes a histone variant that is an integral part of the nucleosome core, playing a critical role in the packaging of DNA into chromatin. Research into HIST2H3A is significant because histone variants are crucial for regulating gene expression, DNA repair, and chromosomal stability. Unlike conventional histones, HIST2H3A is implicated in specific gene regulation mechanisms, cellular differentiation, and response to environmental stimuli. Its dysregulation has been associated with various diseases, including cancer, where improper histone modification patterns contribute to tumorigenesis. Understanding HIST2H3A through recombinant protein studies allows researchers to investigate its structural properties, interactions with DNA, and functional implications in cellular contexts. Furthermore, recombinant HIST2H3A can serve as a valuable tool for studying epigenetic modifications and chromatin dynamics, providing insights into how histone modifications influence gene transcription and cellular behavior. This research has broad implications for understanding the fundamental processes of cellular biology and the development of potential therapeutic strategies aimed at correcting epigenetic dysregulation in diseases.
