Analytical Data
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Gene name
HIST1H4A
- Application
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Alternative Names
HIST1H4A；H4/A；H4FA；HIST1H4A；Histone H4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P62805
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Expression Region
2-103aa
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AA Sequence
SGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG FGG
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HIST1H4A, also known as Histone H4, is a core component of the nucleosome, playing a crucial role in the organization and packaging of DNA within the chromatin structure of eukaryotic cells. This protein is part of the histone family, which comprises proteins that facilitate the formation of chromatin and are fundamental in processes such as gene regulation, DNA replication, and repair. Recent studies have highlighted the significance of HIST1H4A and its post-translational modifications in various biological processes, including epigenetic regulation and cellular response to environmental signals. Furthermore, aberrant expression of histone proteins, including HIST1H4A, has been implicated in various diseases, especially cancers, where alterations in chromatin structure can lead to misregulation of gene expression. Understanding the functional dynamics of HIST1H4A through recombinant protein studies is crucial for deciphering its role in chromatin biology and its potential as a therapeutic target. Researchers are increasingly focusing on producing recombinant HIST1H4A to elucidate its structural properties and interactions with DNA and other chromatin-associated proteins. This research not only enhances our comprehension of histone biology but also opens avenues for developing novel strategies in cancer treatment and epigenetic therapies. By studying the properties and functions of HIST1H4A, scientists aim to uncover the underlying mechanisms governing gene expression and chromatin remodeling, thereby contributing to the broader fields of molecular biology and therapeutic innovation.
