Analytical Data
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Gene name
USP3
- Application
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Alternative Names
Deubiquitinating enzyme 3; EC 3.1.2.15; SIH003; Ubiquitin carboxyl-terminal hydrolase 3; Ubiquitin specific peptidase 3; Ubiquitin specific processing protease 3; Ubiquitin Specific Protease 3; Ubiquitin thioesterase 3; Ubiquitin thiolesterase 3; Ubiquitin-specific-processing protease 3; UBP; UBP3_HUMAN; Usp3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y6I4
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Expression Region
1-520 aa
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AA Sequence
MECPHLSSSV CIAPDSAKFP NGSPSSWCCS VCRSNKSPWV CLTCSSVHCG RYVNGHAKKH YEDAQVPLTN HKKSEKQDKV QHTVCMDCSS YSTYCYRCDD FVVNDTKLGL VQKVREHLQN LENSAFTADR HKKRKLLENS TLNSKLLKVN GSTTAICATG LRNLGNTCFM NAILQSLSNI EQFCCYFKEL PAVELRNGKT AGRRTYHTRS QGDNNVSLVE EFRKTLCALW QGSQTAFSPE SLFYVVWKIM PNFRGYQQQD AHEFMRYLLD HLHLELQGGF NGVSRSAILQ ENSTLSASNK CCINGASTVV TAIFGGILQN EVNCLICGTE SRKFDPFLDL SLDIPSQFRS KRSKNQENGP VCSLRDCLRS FTDLEELDET ELYMCHKCKK KQKSTKKFWI QKLPKVLCLH LKRFHWTAYL RNKVDTYVEF PLRGLDMKCY LLEPENSGPE SCLYDLAAVV VHHGSGVGSG HYTAYATHEG RWFHFNDSTV TLTDEETVVK AKAYILFYVE HQAKAGSDKL
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Molecular Weight
58.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
USP3 (ubiquitin-specific protease 3) is a deubiquitinating enzyme that plays a crucial role in regulating cellular processes by removing ubiquitin moieties from target proteins, thereby influencing their stability, activity, and localization. Research on USP3 has garnered significant interest due to its involvement in various biological pathways, including DNA damage response, cell cycle regulation, and immune responses. Dysregulation of USP3 has been implicated in several diseases, such as cancer and neurodegenerative disorders, making it a promising target for therapeutic intervention. The recombinant production of USP3 has enabled researchers to obtain large quantities of the protein, facilitating in-depth studies of its enzymatic properties and interactions with other cellular components. By elucidating the specific mechanisms by which USP3 exerts its effects, scientists aim to provide insights into potential biomarkers and novel treatment strategies for diseases associated with abnormal ubiquitination processes. The ongoing investigation of USP3's structure-function relationship highlights its importance in cellular homeostasis and its potential as a molecular target in drug development.
