Analytical Data
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Gene name
FBXO47
- Application
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Alternative Names
FBXO47F-box only protein 47
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5MNV8
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Expression Region
1-452aa
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AA Sequence
MASRINTNFT LIPNQKLRRS NRQTSCYSKT LGSGFQPIST FGNFKALPLE IFQIILKYLS VKDISMLSMV SKTVSQHIIN YISTSSGSKR LLLQDFHNLE LPDRRQDSAI LEHYRSLGLL FKRCTLLLPT KERLKYIHKI LTEVSCFKFN GCAAPMQCLG LTCYGMFLQT LTAGWDELEC HRVYNFLCEL TNLCRKIQMA VCSKPGSAQK LELRIRLFCR NVLLDHWTHR SDSAFWLTRI LKPWPMVNQA RLLYIIFGPI SPQDGQVVWQ EMIEEPTDEF SLKGLADAIK LLYDASTKEW TADDVISLVD ELSVVPREWL LENNARLLML SGNNICFSFM ASKAVNGRTI ELARLVVFLA LVCEKELYCM DWTVKMMQKV CKVFSTPVER KNFLQNVANA FACVIMEMLQ SIMSGDRDED DRSFLNLFHL VHAQANFHKE VLYLTMNTPL ST
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Molecular Weight
51.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FBXO47 is a member of the F-box protein family, which plays a crucial role in the ubiquitin-proteasome degradation pathway. This pathway is vital for regulating protein levels within cells and thus controlling various cellular processes, including cell cycle progression, signal transduction, and stress responses. FBXO47 specifically interacts with substrates containing phosphorylated residues, marking them for ubiquitination and subsequent degradation. Recent research has highlighted its involvement in several physiological and pathological processes, including embryonic development, tissue homeostasis, and cancer progression. FBXO47 has been shown to regulate tumor suppressor proteins and oncogenes, suggesting a pivotal role in cancer biology. The study of FBXO47 recombinant proteins has enabled the elucidation of its structure-function relationship, cellular localization, and interaction with other cellular pathways. Investigating FBXO47's mechanism of action could provide insights into novel therapeutic targets for diseases where protein misregulation is a hallmark, particularly in malignancies. As such, the exploration of FBXO47-recombinant proteins is not only essential for understanding its biological functions but also for the development of innovative strategies to manipulate its activities for therapeutic benefits.
