Analytical Data
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Gene name
GFPT2
- Application
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Alternative Names
GFPT2；Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O94808
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Expression Region
2-682aa
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AA Sequence
CGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVK ERHIQLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS AVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSVH YPGEAVATRR GSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK RLDSSACLHA VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGVTNT VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL QNRRQEIIRG LRSLPELIKE VLSLEEKIHD LALELYTQRS LLVMGRGYNY ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GFPT2 (Glutamine Fructose-6-Phosphate Transaminase 2) is an important enzyme involved in the hexosamine biosynthetic pathway, which plays a crucial role in cellular metabolism and nutrient sensing. In this pathway, fructose-6-phosphate is converted into glucosamine-6-phosphate, a precursor for the synthesis of glycoproteins and glycolipids, essential for various cellular functions. Research has shown that GFPT2 is implicated in various biological processes, including insulin signaling, cell proliferation, and apoptosis, making it a potential target for therapeutic interventions in metabolic disorders and cancers. Elevated levels of GFPT2 have been associated with insulin resistance and type 2 diabetes, highlighting its role in the alteration of metabolic pathways related to glucose and fatty acid metabolism. Moreover, GFPT2 modulates the production of O-GlcNAcylation, a post-translational modification that influences protein stability and function. As a result, understanding the structure and function of GFPT2 through recombinant protein studies can provide valuable insights into its biochemical mechanisms, regulatory roles, and potential as a biomarker or drug target in metabolic and malignant diseases. The research on GFPT2 is advancing rapidly, with recombinant protein techniques facilitating detailed investigations into its enzymatic activity, interaction with other proteins, and influence on cellular signaling pathways, paving the way for innovative therapeutic strategies.
