Analytical Data
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基因名
GFPT2
- Application
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别名
GFPT2；Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O94808
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表达区间
2-682aa
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氨基酸序列
CGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVK ERHIQLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS AVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSVH YPGEAVATRR GSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK RLDSSACLHA VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGVTNT VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL QNRRQEIIRG LRSLPELIKE VLSLEEKIHD LALELYTQRS LLVMGRGYNY ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GFPT2 (Glutamine Fructose-6-Phosphate Transaminase 2) is an important enzyme involved in the hexosamine biosynthetic pathway, which plays a crucial role in cellular metabolism and nutrient sensing. In this pathway, fructose-6-phosphate is converted into glucosamine-6-phosphate, a precursor for the synthesis of glycoproteins and glycolipids, essential for various cellular functions. Research has shown that GFPT2 is implicated in various biological processes, including insulin signaling, cell proliferation, and apoptosis, making it a potential target for therapeutic interventions in metabolic disorders and cancers. Elevated levels of GFPT2 have been associated with insulin resistance and type 2 diabetes, highlighting its role in the alteration of metabolic pathways related to glucose and fatty acid metabolism. Moreover, GFPT2 modulates the production of O-GlcNAcylation, a post-translational modification that influences protein stability and function. As a result, understanding the structure and function of GFPT2 through recombinant protein studies can provide valuable insights into its biochemical mechanisms, regulatory roles, and potential as a biomarker or drug target in metabolic and malignant diseases. The research on GFPT2 is advancing rapidly, with recombinant protein techniques facilitating detailed investigations into its enzymatic activity, interaction with other proteins, and influence on cellular signaling pathways, paving the way for innovative therapeutic strategies.
