Cat: PAX2000-12874

Recombinant Human ZNF446 Protein,GST

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Analytical Data

  • Gene name

    ZNF446

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    ZNF446; ZKSCAN20Zinc finger Protein 446; Zinc finger Protein with KRAB and SCAN domains 20

  • Species

    Human

  • Source

    E. coli

  • Tag

    GST-tag at N-terminal

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9NWS9

  • Expression Region

    1-450 aa

  • AA Sequence

    MPSPLGPPCLPVMDPETTLEEPETARLRFRGFCYQEVAGPREALARLRELCCQWLQPEAHSKEQMLEMLVLEQFLGTLPPEIQAWVRGQRPGSPEEAAALVEGLQHDPGQLLGWITAHVLKQEVLPAAQKTEEPLGSPHPSGTVESPGEGPQDTRIEGSVQLSCSVKEEPNVDGQEVAPSSPPLAAQSPEGNHGHQEPASTSFHPPRIQEEWGLLDRSQKELYWDAMLEKYGTVVSLGLPPHQPEAQAQSELGMLLTGTGVCRSLRSGNESEGPPGCPEAQPPQGPGPAAWEGLSGAATPAPTVRPGTPPVPTQPTPAETRLEPAATPRKPYTCEQCGRGFDWKSVFVIHHRTHTSGPGVQSPGLATGESTEKPPQGEVAFPHHPRRSLTGPRSYPCEECGCSFSWKSQLVIHRKSHTGQRRHFCSDCGRAFDWKSQLVIHRKGHRPEVP

  • Molecular Weight

    75.4 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

ZNF446, a member of the Krüppel-type zinc finger protein family, has garnered attention in recent years due to its potential roles in transcriptional regulation, cellular differentiation, and development. Studies suggest that ZNF446 may play a critical role in various biological processes, including stem cell maintenance and tissue homeostasis, owing to its ability to interact with DNA and other proteins within the nucleus. Dysregulation of ZNF446 expression has been implicated in several diseases, including cancer, highlighting its importance as a potential biomarker and therapeutic target. The exploration of ZNF446 recombinant protein is crucial for elucidating its functional mechanisms, enabling the study of its binding affinities and interactions with specific DNA sequences or other protein partners. This knowledge could enhance our understanding of gene regulatory networks and contribute to the development of novel strategies for disease intervention. Thus, the production and characterization of ZNF446 recombinant protein represent a significant step towards unraveling its biological significance and therapeutic potential.

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