Analytical Data
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Gene name
EVI5
- Application
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Alternative Names
EVI5; NB4SEcotropic viral integration site 5 protein homolog; EVI-5; Neuroblastoma stage 4S gene protein
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60447
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Expression Region
1-810aa
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AA Sequence
MVTNKMTAAFRNPSGKQVATDKVAEKLSSTLSWVKNTVSHTVSQMASQVASPSTSLHTTSSSTTLSTPALSPSSPSQLSPDDLELLAKLEEQNRLLETDSKSLRSVNGSRRNSGSSLVSSSSASSNLSHLEEDSWILWGRIVNEWEDVRKKKEKQVKELVHKGIPHHFRAIVWQLLCSAQSMPIKDQYSELLKMTSPCEKLIRRDIARTYPEHNFFKEKDSLGQEVLFNVMKAYSLVDREVGYCQGSAFIVGLLLMQMPEEEAFCVFVKLMQDYRLRELFKPSMAELGLCMYQFECMIQEHLPELFVHFQSQSFHTSMYASSWFLTIFLTTFPLPIATRIFDIFMSEGLEIVFRVGLALLQMNQAELMQLDMEGMLQHFQKVIPHQFDGVPDKLIQAAYQVKYNSKKMKKLEKEYTTIKTKEMEEQVEIKRLRTENRLLKQRIETLEKHKCSSNYNEDFVLQLEKELVQARLSEAESQCALKEMQDKVLDIEKRNNSLPDENNIARLQEELIAVKLREAEAIMGLKELRQQVKDLEEHWQRHLARTTGRWKDPPKKNAMNELQDELMTIRLREAETQAEIREIKQRMMEMETQNQINSNHLRRAEQEVISLQEKVQYLSAQNKGLLTQLSEAKRKQAEIECKNKEEVMAVRLREADSIAAVAELRQHIAELEIQKEEGKLQGQLNKSDSNQYIGELKDQIAELNHELRCLKGQRGFSGQPPFDGIHIVNHLIGDDESFHSSDEDFIDNSLQETGVGFPLHGKSGSMSLDPAVADGSESETEDSVLETRESNQVVQKERPPRRRESYSTTV
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Molecular Weight
115.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
EVI5, or “Ecotropic Viral Integration site 5”, is a gene that has garnered attention in cancer research due to its role in cell proliferation and differentiation. Studies have shown that EVI5 is frequently overexpressed in various malignancies, suggesting its potential function as an oncogene. The protein encoded by EVI5 is thought to be involved in the regulation of cell signaling pathways, particularly those associated with the endosomal and lysosomal trafficking system, which is crucial for various cellular processes, including receptor recycling and degradation. Understanding the structure and function of the EVI5 protein through recombinant expression systems is essential for elucidating its role in tumorigenesis. Research into EVI5 has also highlighted its involvement in immune response modulation, making it a candidate for therapeutic targeting. Advances in recombinant protein technology allow researchers to produce EVI5 in vitro, enabling detailed biochemical studies and the development of potential inhibitors. Investigating EVI5's molecular mechanisms not only enhances the understanding of tumor biology but also opens avenues for novel cancer therapies, including the potential use of EVI5 as a biomarker for cancer diagnosis and prognosis. Consequently, the ongoing research on EVI5 recombinant protein is critical for unraveling its functions in both normal physiology and disease states, ultimately contributing to the broader understanding of cancer biology and therapeutic strategies.
