Analytical Data
-
基因名
DLAT
- Application
-
别名
DLAT；PDHK3；[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3. mitochondrial
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P10515
-
表达区间
93-640aa
-
氨基酸序列
KVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVPPTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDIDSFVPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLE
-
分子量
62.2kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DLAT, or dihydrolipoamide acyltransferase, is a crucial component of the pyruvate dehydrogenase complex (PDC), which plays a significant role in cellular metabolism by linking glycolysis to the Krebs cycle. Research into DLAT recombinant protein has garnered attention due to its potential implications in metabolic disorders, including diabetes and obesity. The reconstitution of DLAT as a recombinant protein allows for a detailed investigation into its functional properties, post-translational modifications, and interactions with other metabolic enzymes. Understanding the structure-function relationship of DLAT could lead to insights into its regulatory mechanisms and its role in metabolic pathways. Furthermore, studying DLAT in a recombinant form facilitates the development of targeted therapeutics aimed at modulating its activity, which may have potential applications in treating diseases associated with disrupted energy metabolism. As the prevalence of metabolic diseases continues to rise globally, research on DLAT and its recombinant forms is increasingly relevant, presenting opportunities for innovative therapeutic strategies to enhance metabolic health and prevent associated complications.
