Analytical Data
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基因名
DGAT1
- Application
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别名
DGAT1；AGRP1；DGAT；Diacylglycerol O-acyltransferase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75907
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表达区间
240-488aa
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氨基酸序列
TVSYPDNLTYRDLYYFLFAPTLCYELNFPRSPRIRKRFLLRRILEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIWLIFFYWLFHSCLNAVAELMQFGDREFYRDWWNSESVTYFWQNWNIPVHKWCIRHFYKPMLRRGSSKWMARTGVFLASAFFHEYLVSVPLRMFRLWAFTGMMAQIPLAWFVGRFFQGNYGNAAVWLSLIIGQPIAVLMYVHDYYVLNYEAPAAEA
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分子量
37.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DGAT1 (diacylglycerol O-acyltransferase 1) is a key enzyme involved in triglyceride biosynthesis, playing a crucial role in lipid metabolism and energy storage. It catalyzes the final step in the conversion of diacylglycerol and acyl-CoA into triacylglycerol, a process vital for maintaining cellular lipid homeostasis. Dysregulation of DGAT1 activity has been implicated in various metabolic disorders, including obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Given its importance in lipid metabolism, DGAT1 has emerged as a promising therapeutic target for the treatment of metabolic diseases. Research on recombinant DGAT1 protein has focused on elucidating its structure, function, and regulatory mechanisms. The production of this enzyme in a recombinant form allows for detailed biochemical studies, including characterization of its catalytic activity and interaction with various substrates and inhibitors. Moreover, this research has provided insights into the enzyme's role in lipid droplet formation and its potential effects on systemic lipid levels. Understanding DGAT1's functionality and regulation is critical for developing targeted therapies aimed at modulating its activity to treat metabolic disorders effectively, highlighting the significance of DGAT1 recombinant protein studies in both basic research and clinical applications.
