Analytical Data
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基因名
DGAT2
- Application
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别名
DGAT2；Diacylglycerol O-acyltransferase 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96PD7
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表达区间
1-388aa
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氨基酸序列
MKTLIAAYSGVLRGERQAEADRSQRSHGGPALSREGSGRWGTGSSILSALQDLFSVTWLNRSKVEKQLQVISVLQWVLSFLVLGVACSAILMYIFCTDCWLIAVLYFTWLVFDWNTPKKGGRRSQWVRNWAVWRYFRDYFPIQLVKTHNLLTTRNYIFGYHPHGIMGLGAFCNFSTEATEVSKKFPGIRPYLATLAGNFRMPVLREYLMSGGICPVSRDTIDYLLSKNGSGNAIIIVVGGAAESLSSMPGKNAVTLRNRKGFVKLALRHGADLVPIYSFGENEVYKQVIFEEGSWGRWVQKKFQKYIGFAPCIFHGRGLFSSDTWGLVPYSKPITTVVGEPITIPKLEHPTQQDIDLYHTMYMEALVKLFDKHKTKFGLPETEVLEVN
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DGAT2 (Diacylglycerol O-acyltransferase 2) is an enzyme crucial for triglyceride biosynthesis, playing a significant role in lipid metabolism and energy storage within cells. The enzyme catalyzes the final step of triacylglycerol formation, converting diacylglycerol and acyl-CoA into triglycerides, which are essential for maintaining cellular energy balance. Understanding the structure and function of DGAT2 is pivotal due to its implications in metabolic disorders and obesity, as dysregulation of lipid metabolism can lead to conditions such as non-alcoholic fatty liver disease (NAFLD) and cardiovascular diseases. Overexpression or inhibition of DGAT2 has been shown to influence fat accumulation in various tissues, making it a potential therapeutic target for treating metabolic syndromes. Recent studies focus on the recombinant expression of DGAT2 to examine its enzymatic properties, interaction with other proteins, and potential role in lipid droplet formation. By utilizing recombinant technology, researchers aim to gain insights into the enzyme’s structure-function relationship, facilitating the development of selective inhibitors or activators that could serve as novel treatments for obesity and related metabolic disorders. This research area continues to grow, emphasizing the need for efficient methods of producing high-quality DGAT2 protein for in vitro studies and potential drug design applications.
