Cat: PA2000-7369

Recombinant Human EP400NL Protein,GST

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Analytical Data

  • Gene name

    EP400NL

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    EP400P1; EP400NL; Putative EP400-like protein; EP400 pseudogene 1

  • Species

    Human

  • Source

    E. coli

  • Tag

    GST-tag at N-terminal

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q6ZTU2

  • Expression Region

    1-419aa

  • AA Sequence

    MQHVSSSQSSQRHVQWPGACPGAGEEQPACSQPSLPLTLPSPSHQLQQLMVQTQSPTQPSPGPGQALQNVRAGAPGPGLGLCSSSPTGDFVDASVLVRQISLSPSSGGHFVFQDGSGLTQIAQGAQVQLQHPGTPITVRERRPSQPHTQSGGTIHHLGPQSPAAAGGAGLQPLASPSHITTANLPPQISSIIQGQLVQQQQVLQGPPLPRPLGFERTPGVLLPGAGGAAGFGMTSPPPPTSPSRTAVPPGLSSLPLTSVGNTGMKKVPKKLEEIPPASPEMAQMRKQCLDYHHQEMQALKEVFKEYLIELFFLQHFQGNMMDFLAFKERLYGPLQAYLRQNDLDIEEEEEEHFEVINDEVKVVARKHGQPGTPVAIATQLPPRTSAAFPAQQQPLQVLSDGSTVQLPRLSSLGFEDSMC

  • Molecular Weight

    71 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

EP400NL is a member of the E3 ubiquitin ligase family, which plays a critical role in various cellular processes, including protein degradation, signaling pathways, and cellular response to stress. Research on EP400NL has gained prominence due to its involvement in the regulation of gene expression and its association with several diseases, including cancer and neurodegenerative disorders. The protein has been shown to facilitate the recruitment of ubiquitin-conjugating enzymes to target proteins, thereby influencing their stability and function. Recent studies suggest that EP400NL may also play a role in chromatin remodeling and transcriptional regulation, which are essential for maintaining cellular homeostasis. Understanding the functional mechanisms of EP400NL and its interactions within the ubiquitin-proteasome system is crucial for elucidating its potential as a therapeutic target. Further research may lead to the development of novel strategies for treating diseases associated with dysregulated ubiquitination processes, making EP400NL a promising candidate for targeted therapies in molecular medicine.

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