Analytical Data
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Gene name
ENTPD8
- Application
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Alternative Names
ENTPD8; UNQ2492/PRO5779Ectonucleoside triphosphate diphosphohydrolase 8; E-NTPDase 8; NTPDase 8; NTPDase8; EC 3.6.1.5
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5MY95
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Expression Region
1-458aa
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AA Sequence
MGLSRKEQVFLALLGASGVSGLTALILLLVEATSVLLPTDIKFGIVFDAGSSHTSLFLYQWLANKENGTGVVSQALACQVEGPGISSYTSNAAQAGESLQGCLEEALVLIPEAQHRKTPTFLGATAGMRLLSRKNSSQARDIFAAVTQVLGRSPVDFWGAELLAGQAEGAFGWITVNYGLGTLVKYSFTGEWIQPPEEMLVGALDMGGASTQITFVPGGPILDKSTQADFRLYGSDYSVYTHSYLCFGRDQMLSRLLVGLVQSRPAALLRHPCYLSGYQTTLALGPLYESPCVHATPPLSLPQNLTVEGTGNPGACVSAIRELFNFSSCQGQEDCAFDGVYQPPLRGQFYVEASYPGQDRWLRDYCASGLYILTLLHEGYGFSEETWPSLEFRKQAGGVDIGWTLGYMLNLTGMIPADAPAQWRAESYGVWVAKVVFMVLALVAVVGAALVQLFWLQD
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Molecular Weight
77.33 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ENTPD8, a member of the ecto-nucleotide triphosphate diphosphohydrolase family, has gained significant interest in recent years due to its potential roles in various physiological and pathological processes. Located primarily in the cell membrane, ENTPD8 is involved in the hydrolysis of nucleotides, specifically ATP and ADP, which play crucial roles in cell signaling and energy metabolism. Its enzymatic activity has implications in regulating purinergic signaling pathways, influencing cell proliferation, differentiation, and apoptosis. Abnormal expression or activity of ENTPD8 has been linked to several diseases, including cancer and neurodegenerative disorders, underscoring its potential as a therapeutic target. Moreover, the structural and functional characterization of ENTPD8 can provide insights into its unique substrate specificity and regulatory mechanisms, which may differ from other ecto-nucleotide hydrolases. Research focusing on the recombinant expression of ENTPD8 allows for detailed studies of its enzymatic properties and biological functions, facilitating the development of specific inhibitors or modulators that could be used in therapeutic applications. As such, understanding ENTPD8’s role in cellular processes may pave the way for novel strategies in treating diseases associated with nucleotide metabolism dysregulation, highlighting the importance of further studies in this area.
