Analytical Data
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基因名
PAR2
- Application
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别名
PAR2；GPR11；PAR2；Proteinase-activated receptor 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75469
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表达区间
1-434aa
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氨基酸序列
MEVRPKESWNHADFVHCEDTESVPGKPSVNADEEVGGPQICRVCGDKATGYHFNVMTCEGCKGFFRRAMKRNARLRCPFRKGACEITRKTRRQCQACRLRKCLESGMKKEMIMSDEAVEERRALIKRKKSERTGTQPLGVQGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRLPGVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLKVSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMADMSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAAFELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGFQQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFSPDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPAHRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPFATPLMQELFGITGS
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分子量
69.8kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PAR2 (Proteinase-Activated Receptor 2) is a G protein-coupled receptor that plays a pivotal role in various physiological and pathological processes, including inflammation, pain sensation, and tissue repair. It is activated by serine proteases, such as trypsin and mast cell tryptase, which cleave the receptor’s N-terminal domain, leading to a conformational change that initiates intracellular signaling pathways. Research on PAR2 has gained traction due to its involvement in the modulation of pain and itch, particularly in chronic pain conditions where traditional analgesics often fall short. Moreover, PAR2 has been implicated in various diseases, including asthma, arthritis, and cancer, highlighting its potential as a therapeutic target. The recombinant expression of PAR2 allows for detailed studies of its structure, function, and interaction with ligands. Understanding the molecular mechanisms of PAR2 signaling can pave the way for novel approaches in drug development to target PAR2 for therapeutic benefit in chronic inflammatory diseases and pain management. As such, the study of PAR2 recombinant proteins not only enhances our fundamental understanding of this receptor but also offers insights into innovative treatment strategies for a range of debilitating conditions.
