Analytical Data
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Gene name
THEM5
- Application
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Alternative Names
THEM5; ACOT15; Acyl-coenzyme A thioesterase THEM5; Acyl-CoA thioesterase THEM5; EC 3.1.2.2; Acyl-coenzyme A thioesterase 15; Thioesterase superfamily member 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N1Q8
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Expression Region
1-247 aa
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AA Sequence
MIRRCFQVAA RLGHHRGLLE APRILPRLNP ASAFGSSTDS MFSRFLPEKT DLKDYALPNA SWCSDMLSLY QEFLEKTKSS GWIKLPSFKS NRDHIRGLKL PSGLAVSSDK GDCRIFTRCI QVEGQGFEYV IFFQPTQKKS VCLFQPGSYL EGPPGFAHGG SLAAMMDETF SKTAFLAGEG LFTLSLNIRF KNLIPVDSLV VMDVELDKIE DQKLYMSCIA HSRDQQTVYA KSSGVFLQLQ LEEESPQ
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Molecular Weight
27.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The THEM5 protein has garnered significant attention in the field of molecular biology due to its potential role in various cellular processes, particularly in mitochondrial function and cellular stress response. Emerging evidence suggests that THEM5 is involved in the maintenance of mitochondrial integrity and the regulation of apoptosis, making it a focal point for understanding mitochondrial dynamics and related pathologies. Researchers have noted its importance in the context of neurodegenerative diseases, where mitochondrial dysfunction is a key feature. The study of THEM5 also intersects with the broader narrative of proteostasis and the cellular mechanisms that preserve protein homeostasis under stress conditions. As research progresses, the elucidation of THEM5's structure, function, and interaction with other cellular components is expected to provide insights into its role in health and disease, paving the way for potential therapeutic interventions targeting mitochondrial-related disorders. Furthermore, the exploration of THEM5's regulatory mechanisms holds promise in the development of strategies aimed at enhancing mitochondrial resilience, thereby providing critical information for the advancement of treatment options in conditions characterized by mitochondrial dysfunction.
