Analytical Data
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Gene name
THEM4
- Application
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Alternative Names
Acyl CoA thioesterase THEM4; Acyl coenzyme A thioesterase THEM4; C terminal modulator Protein; Carboxyl-terminal modulator Protein; CTMP; FLJ27206; MGC29636; OTTHUMP00000015248; Them4; THEM4_HUMAN; Thioesterase superfamily member 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5T1C6
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Expression Region
37-240 aa
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AA Sequence
SSEE VILKDCSVPN PSWNKDLRLL FDQFMKKCED GSWKRLPSYK RTPTEWIQDF KTHFLDPKLM KEEQMSQAQL FTRSFDDGLG FEYVMFYNDI EKRMVCLFQG GPYLEGPPGF IHGGAIATMI DATVGMCAMM AGGIVMTANL NINYKRPIPL CSVVMINSQL DKVEGRKFFV SCNVQSVDEK TLYSEATSLF IKLNPAKSLT
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Molecular Weight
27.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
THEM4 (threonyl-tRNA synthetase-interacting multifunctional protein 4) is a relatively newly identified protein that has garnered interest due to its crucial roles in various cellular processes. Research has indicated that THEM4 is involved in the regulation of lipid metabolism, influencing the synthesis and degradation of fatty acids and triglycerides, which plays a vital role in maintaining cellular energy homeostasis. Moreover, THEM4 has been linked to cellular stress responses, particularly in the context of endoplasmic reticulum (ER) stress, where it appears to assist in protein folding and mitigate apoptosis. Studies have also suggested that THEM4 might have implications in metabolic disorders and diseases such as obesity, diabetes, and cardiovascular diseases, highlighting its potential as a therapeutic target. The recombinant expression and characterization of THEM4 have become pivotal for understanding its biological functions and mechanisms at the molecular level. By producing THEM4 in a recombinant system, researchers can explore its structure-function relationships and interactions with other proteins, paving the way for novel interventions in diseases associated with dysregulated lipid metabolism and cellular stress responses. Moreover, the insights gained from THEM4 studies can enhance our understanding of the broader implications of protein regulation in health and disease, emphasizing the importance of continuing research in this area. Overall, the exploration of THEM4 as a recombinant protein provides a promising avenue for advancing biomedicine and understanding complex metabolic pathways.
