Analytical Data
-
Gene name
PCMT1
- Application
-
Alternative Names
PCMT1；Protein-L-isoaspartate(D-aspartate) O-methyltransferase
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P2206
-
Expression Region
1-227aa
-
AA Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK
-
Molecular Weight
29 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PCMT1 (Protein L-isoaspartate O-methyltransferase 1) is an enzyme that plays a critical role in the repair and maintenance of damaged proteins. It catalyzes the methylation of L-isoaspartate residues, which are formed during the spontaneous deamidation of aspartate and asparagine in aging and stressed proteins. The accumulation of these damaged proteins is associated with various age-related diseases, including neurodegenerative disorders like Alzheimer's disease. Understanding the biological functions of PCMT1 and its enzyme mechanisms is essential for developing therapeutic strategies targeting the accumulation of damaged proteins. Additionally, recombinant PCMT1 protein serves as a valuable tool for structural and functional studies that may reveal insights into its activity and regulation. Researchers have focused on the expression and purification of PCMT1 for biochemical assays, aiming to delineate its role in cellular repair processes and its potential involvement in disease progression. By investigating PCMT1, scientists hope to develop interventions that could enhance protein quality control and mitigate the pathological effects of protein misfolding and aggregation, ultimately contributing to improved health outcomes in aging populations.
