Analytical Data
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Gene name
POMT1
- Application
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Alternative Names
POMT1；Protein O-mannosyl-transferase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y6A1
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Expression Region
1-747aa
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AA Sequence
MWGFLKRPVVVTADINLSLVALTGMGLLSRLWRLTYPRAVVFDEVYYGQYISFYMKQIFFLDDSGPPFGHMVLALGGYLGGFDGNFLWNRIGAEYSSNVPVWSLRLLPALAGALSVPMAYQIVLELHFSHCAAMGAALLMLIENALITQSRLMLLESVLIFFNLLAVLSYLKFFNCQKHSPFSLSWWFWLTLTGVACSCAVGIKYMGVFTYVLVLGVAAVHAWHLLGDQTLSNVGADVQCCMRPACMGQMQMSQGVCVFCHLLARAVALLVIPVVLYLLFFYVHLILVFRSGPHDQIMSSAFQASLEGGLARITQGQPLEVAFGSQVTLRNVFGKPVPCWLHSHQDTYPMIYENGRGSSHQQQVTCYPFKDVNNWWIVKDPRRHQLVVSSPPRPVRHGDMVQLVHGMTTRSLNTHDVAAPLSPHSQEVSCYIDYNISMPAQNLWRLEIVNRGSDTDVWKTILSEVRFVHVNTSAVLKLSGAHLPDWGYRQLEIVGEKLSRGYHGSTVWNVEEHRYGASQEQRERERELHSPAQVDVSRNLSFMARFSELQWRMLALRSDDSEHKYSSSPLEWVTLDTNIAYWLHPRTSAQIHLLGNIVIWVSGSLALAIYALLSLWYLLRRRRNVHDLPQDAWLRWVLAGALCAGGWAVNYLPFFLMEKTLFLYHYLPALTFQILLLPVVLQHISDHLCRSQLQRSIFSALVVAWYSSACHVSNTLRPLTYGDKSLSPHELKALRWKDSWDILIRKH
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of POMT1 (Protein O-Mannosyltransferase 1) recombinant protein has gained significant attention due to its crucial role in glycoprotein biosynthesis and its connection to various genetic disorders. POMT1 is an enzyme involved in the post-translational modification of proteins, specifically in the addition of mannose to serine and threonine residues on nascent polypeptides. Mutations in the POMT1 gene have been linked to a group of congenital muscular dystrophies, including Walker-Warburg syndrome and muscle-eye-brain disease, which are characterized by muscular weakness, brain malformations, and ocular defects. Understanding the structure and function of POMT1 is essential for elucidating the molecular mechanisms underlying these diseases. The development of recombinant POMT1 proteins permits in-depth studies of its enzymatic activity, substrate specificity, and interactions with other cellular components. Moreover, these recombinant proteins can serve as valuable tools for drug discovery and therapeutic interventions aimed at ameliorating the effects of POMT1-related disorders. The research surrounding POMT1 recombinant proteins contributes not only to the understanding of muscle and neurological pathologies but also enhances our knowledge of protein glycosylation, a fundamental process with implications across various fields such as cell biology, immunology, and biotechnology. Consequently, ongoing efforts to produce and characterize POMT1 in a recombinant system hold promise for advancing both basic research and clinical applications, paving the way for potential treatments and improved patient outcomes in congenital glycosylation disorders.
