Analytical Data
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Gene name
DNMT2
- Application
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Alternative Names
dDNMT; DmMT 2; DmMT2; DNA (cytosine 5 ) methyltransferase 2; DNA (cytosine 5) methyltransferase like protein 2; DNA (cytosine-5)-methyltransferase-like protein 2; DNA 5 cytosine methyltransferase; DNA methyltransferase 2; DNA methyltransferase homolog HsaIIP; DNA MTase homolog HsaIIP; Dnmt 2; Dnmt2; M.HsaIIP
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14717
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Expression Region
1-391aa
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AA Sequence
MEPLRVLELY SGVGGMHHAL RESCIPAQVV AAIDVNTVAN EVYKYNFPHT QLLAKTIEGI TLEEFDRLSF DMILMSPPCQ PFTRIGRQGD MTDSRTNSFL HILDILPRLQ KLPKYILLEN VKGFEVSSTR DLLIQTIENC GFQYQEFLLS PTSLGIPNSR LRYFLIAKLQ SEPLPFQAPG QVLMEFPKIE SVHPQKYAMD VENKIQEKNV EPNISFDGSI QCSGKDAILF KLETAEEIHR KNQQDSDLSV KMLKDFLEDD TDVNQYLLPP KSLLRYALLL DIVQPTCRRS VCFTKGYGSY IEGTGSVLQT AEDVQVENIY KSLTNLSQEE QITKLLILKL RYFTPKEIAN LLGFPPEFGF PEKITVKQRY RLLGNSLNVH VVAKLIKILY E
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Molecular Weight
44.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DNMT2, or DNA methyltransferase 2, is a member of the DNA methyltransferase family, traditionally associated with the regulation of gene expression through DNA methylation. Historically, it was thought that DNMTs primarily functioned in mammals; however, DNMT2 has garnered attention in the study of various organisms, including plants and invertebrates, suggesting a broader role in epigenetic regulation. Unlike its counterparts that methylate cytosines in mammalian DNA, DNMT2 was shown to exhibit tRNA methyltransferase activity, highlighting its involvement in tRNA processing and stability. This function implicates DNMT2 in the maintenance of cellular homeostasis and the modulation of various biological processes, such as stress response and developmental cues. Recent studies have also indicated its potential association with certain diseases, underscoring the significance of understanding DNMT2's structure and function at the molecular level. As researchers explore the full spectrum of DNMT2's roles, the development of recombinant DNMT2 proteins has become a crucial focus, enabling the investigation of its enzymatic activity, substrate specificity, and interactions with other cellular factors. The characterization of these recombinant proteins is expected to pave the way for novel therapeutic approaches targeting DNMT2-related pathways, providing insights into epigenetic regulation across different biological contexts. Thus, unraveling the complexities surrounding DNMT2 stands as a promising frontier in both basic and applied biomedical research, contributing to our comprehension of epigenetic mechanisms and their implications in health and disease.
