Analytical Data
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基因名
CRISP2
- Application
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别名
CRISP2；AEGL1；Cysteine-rich secretory Protein 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P16562
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表达区间
22-243aa
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氨基酸序列
KDPAFTALL TTQLQVQREI VNKHNELRKA VSPPASNMLK MEWSREVTTN AQRWANKCTL QHSDPEDRKT STRCGENLYM SSDPTSWSSA IQSWYDEILD FVYGVGPKSP NAVVGHYTQL VWYSTYQVGC GIAYCPNQDS LKYYYVCQYC PAGNNMNRKN TPYQQGTPCA GCPDDCDKGL CTNSCQYQDL LSNCDSLKNT AGCEHELLKE KCKATCLCEN KIY
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CRISP2, or cysteine-rich secretory protein 2, is a member of the CRISP protein family, which is known for its involvement in various biological processes, including reproduction and immunity. Research has shown that CRISP2 plays a crucial role in the function of sperm, specifically in capacitation, acrosome reaction, and fertilization. Recent studies emphasize its potential involvement in male fertility as it influences sperm-egg interaction and sperm motility. Additionally, CRISP2 has been implicated in certain pathologies, including autoimmune diseases and cancer, due to its role in regulating immune responses and cellular signaling pathways. The recombinant expression of CRISP2 allows for in-depth studies of its structure and function, facilitating the exploration of its biological significance and therapeutic potential. By utilizing recombinant technology, researchers can produce substantial quantities of CRISP2 for various assays and treatments, including fertility interventions and immunological studies. Understanding the function of CRISP2 at a molecular level could provide insights into its role not only in reproductive biology but also in broader physiological and pathological contexts. Consequently, the study of CRISP2 and its recombinant forms presents opportunities to develop novel strategies for diagnosing and treating male infertility and associated diseases.
