Analytical Data
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基因名
WNK1
- Application
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别名
WNK1；HSN2；KDP；KIAA0344；Serine/threonine-Protein kinase WNK1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H4A3
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表达区间
181-507aa
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氨基酸序列
ARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIE
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分子量
67 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WNK1, a member of the WNK (With No K) kinase family, plays a pivotal role in regulating ion homeostasis and blood pressure. Elevated levels of WNK1 have been implicated in several disorders, such as hypertension, heart disease, and certain forms of chronic kidney disease. This kinase is known for its unique regulatory mechanisms, including its interaction with the SPAK and OSR1 proteins, which are crucial in the signaling pathways controlling sodium and potassium transport in renal epithelial cells. Research into WNK1 recombinant proteins aims to elucidate the molecular mechanisms governing its activity and the pathways it influences. Given its involvement in essential physiological processes, WNK1 presents a compelling target for therapeutic intervention. Understanding the structure-function relationship of WNK1 through recombinant technology offers insights into the potential development of specific inhibitors or modulators that could ameliorate the effects of associated pathologies. As such, ongoing studies focus on the expression, purification, and functional characterization of WNK1 recombinant proteins, which could pave the way for innovative treatment strategies targeting the underlying causes of WNK1-related diseases.
