Analytical Data
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Gene name
DMRT3
- Application
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Alternative Names
DMRT3; DMRTA3Doublesex- and mab-3-related transcription factor 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NQL9
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Expression Region
1-472aa
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AA Sequence
MNGYGSPYLY MGGPVSQPPR APLQRTPKCA RCRNHGVLSW LKGHKRYCRF KDCTCEKCIL IIERQRVMAA QVALRRQQAN ESLESLIPDS LRALPGPPPP GDAVAAPQPP PASQPSQPQP PRPAAELAAA AALRWTAEPQ PGALQAQLAK PDLTEERLGD GKSADNTEVF SDKDTDQRSS PDVAKSKGCF TPESPEIVSV EEGGYAVQKN GGNPESRPDS PKCHAEQNHL LIEGPSGTVS LPFSLKANRP PLEVLKKIFP NQKPTVLELI LKGCGGDLVS AVEVLLSSRS SVTGAERTSA EPESLALPSN GHIFEHTLSS YPISSSKWSV GSAFRVPDTL RFSADSSNVV PSPLAGPLQP PFPQPPRYPL MLRNTLARSQ SSPFLPNDVT LWNTMTLQQQ YQLRSQYVSP FPSNSTSVFR SSPVLPARAT EDPRISIPDD GCPFVSKQSI YTEDDYDERS DSSDSRTLNT SS
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Molecular Weight
51.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DMRT3, a member of the Doublesex and Mab-3 Related Transcription factors (DMRT) family, has garnered significant research interest due to its crucial role in sexual development and differentiation in vertebrates. Initially discovered in model organisms, DMRT3 is implicated in the regulation of genes responsible for male-specific traits, and its expression patterns are predominantly observed in the developing gonads and brain. The reorganization of DMRT3 protein is particularly intriguing, as studies have revealed that its splicing variants may contribute to diverse biological functions beyond sex determination, including neural development and behavioral patterns. Recent advances in molecular biology techniques, such as CRISPR-Cas9 and RNA sequencing, have facilitated a deeper understanding of DMRT3’s functional mechanisms and regulatory networks. Furthermore, research indicates that alterations in DMRT3 expression or mutations in its coding regions can lead to reproductive anomalies and other developmental disorders, highlighting its significance in evolutionary biology and potential implications for agricultural and biomedical applications. By studying the recombinant DMRT3 protein, researchers aim to elucidate its structure, binding interactions, and regulatory pathways, thereby providing insights into its multifaceted roles in development and evolution, and potentially offering new approaches for addressing fertility issues and sex-related disorders in various species.
