Analytical Data
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Gene name
DMRT1
- Application
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Alternative Names
CT154; DM domain expressed in testis 1; DM domain expressed in testis protein 1; DM domain gene expressed in testis; DMRT 1; dmrt1; DMRT1_HUMAN; DMT 1; DMT1; Doublesex and mab 3 related transcription factor 1; Doublesex- and mab-3-related transcription factor 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y5R6
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Expression Region
1-373aa
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AA Sequence
MPNDEAFSKPSTPSEAPHAPGVPPQGRAGGFGKASGALVGAASGSSAGGSSRGGGSGSGASDLGAGSKKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCNLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSAAELLVKRENNGSNPCLMTECSGTSQPPPASVPTTAASEGRMVIQDIPAVTSRGHVENTPDLVSDSTYYSSFYQPSLFPYYNNLYNCPQYSMALAADSASGEVGNPLGGSPVKNSLRGLPGPYVPGQTGNQWQMKNMENRHAMSSQYRMHSYYPPPSYLGQSVPQFFTFEDAPSYPEARASVFSPPSSQDSGLVSLSSSSPISNKSTKAVLECEPASEPSSFTVTPVIEEDE
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Molecular Weight
66.77 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DMRT1, or Doublesex and Mab-3 Related Transcription Factor 1, is a critical gene known for its role in sexual determination and differentiation across various species, particularly in vertebrates. It belongs to the DMRT (Doublesex/Mab-3 Related Transcription factor) family, which is crucial for regulating genes that influence sexual development. Research on DMRT1 has gained prominence due to its involvement in both testicular development and ovarian differentiation, making it a key factor in understanding the mechanisms of sex-changing in some fish species and sex determination in reptiles and mammals. Moreover, mutations in the DMRT1 gene have been implicated in disorders of sexual development (DSDs) in humans, prompting investigations into its function and regulatory pathways. Recent advances in recombinant protein technology have allowed researchers to produce DMRT1 in vitro, facilitating structural studies and functional assays that elucidate its interactions with other proteins and DNA. Understanding the precise roles and regulatory mechanisms of DMRT1 is essential not only for developmental biology but also for potential therapeutic interventions in cases of sexual development disorders and evolutionary studies regarding sexual system diversification.
