Analytical Data
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Gene name
PHF8
- Application
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Alternative Names
PHF8；KIAA1111；ZNF422；Histone lysine demethylase PHF8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UPP1
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Expression Region
2-525aa
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AA Sequence
NRSRAIVQR GRVLPPPAPL DTTNLAGRRT LQGRAKMASV PVYCLCRLPY DVTRFMIECD MCQDWFHGSC VGVEEEKAAD IDLYHCPNCE VLHGPSIMKK RRGSSKGHDT HKGKPVKTGS PTFVRELRSR TFDSSDEVIL KPTGNQLTVE FLEENSFSVP ILVLKKDGLG MTLPSPSFTV RDVEHYVGSD KEIDVIDVTR QADCKMKLGD FVKYYYSGKR EKVLNVISLE FSDTRLSNLV ETPKIVRKLS WVENLWPEEC VFERPNVQKY CLMSVRDSYT DFHIDFGGTS VWYHVLKGEK IFYLIRPTNA NLTLFECWSS SSNQNEMFFG DQVDKCYKCS VKQGQTLFIP TGWIHAVLTP VDCLAFGGNF LHSLNIEMQL KAYEIEKRLS TADLFRFPNF ETICWYVGKH ILDIFRGLRE NRRHPASYLV HGGKALNLAF RAWTRKEALP DHEDEIPETV RTVQLIKDLA REIRLVEDIF QQNVGKTSNI FGLQRIFPAG SIPLTRPAHS TSVSMSRLSL PSKNG
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Molecular Weight
62 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PHF8 (PHD-finger protein 8) is a member of the PHD-finger family of proteins and plays a significant role in regulating gene expression through epigenetic mechanisms. It functions as a histone demethylase, specifically targeting dimethylated lysine 9 on histone H3, which is crucial for transcriptional activation. Its dysregulation has been associated with various diseases, including certain cancers and intellectual disabilities. Research on PHF8 has gained momentum owing to its essential functions in development and cellular differentiation, as well as its involvement in the maintenance of stem cell pluripotency. Understanding the structural and functional properties of recombinant PHF8 protein is vital for elucidating its biological roles and interactions. Recombinant techniques allow for the production of PHF8 in a controlled environment, enabling detailed studies on its enzymatic activity, substrate specificity, and molecular interactions. Insights gained from these studies contribute to our understanding of epigenetic regulation mechanisms and may lead to the development of therapeutic strategies targeting PHF8-related pathologies. Given the growing interest in epigenetic factors in health and disease, PHF8 stands out as a promising candidate for further investigation, with the potential to uncover novel insights into gene regulation and its implications in various biological processes.
