Analytical Data
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基因名
DHX32
- Application
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别名
3110079L04Rik; AA408140; Ddx32; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32; DEAD/H box 32; DEAD/H helicase like protein 1; DEAD/H helicase-like protein 1; DEAH (Asp-Glu-Ala-His) box polypeptide 32; DEAH box protein 32; DHLP1; DHX32; DHX32_HUMAN
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q7L7V1
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表达区间
1-743aa
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氨基酸序列
MEEEGLECPNSSSEKRYFPESLDSSDGDEEEVLACEDLELNPFDGLPYSSRYYKLLKEREDLPIWKEKYSFMENLLQNQIVIVSGDAKCGKSAQVPQWCAEYCLSIHYQHGGVICTQVHKQTVVQLALRVADEMDVNIGHEVGYVIPFENCCTNETILRYCTDDMLQREMMSNPFLGSYGVIILDDIHERSIATDVLLGLLKDVLLARPELKLIINSSPHLISKLNSYYGNVPVIEVKNKHPVEVVYLSEAQKDSFESILRLIFEIHHSGEKGDIVVFLACEQDIEKVCETVYQGSNLNPDLGELVVVPLYPKEKCSLFKPLDETEKRCQVYQRRVVLTTSSGEFLIWSNSVRFVIDVGVERRKVYNPRIRANSLVMQPISQSQAEIRKQILGSSSSGKFFCLYTEEFASKDMTPLKPAEMQEANLTSMVLFMKRIDIAGLGHCDFMNRPAPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILASCEFDCVDEVLTIAAMVTAPNCFSHVPHGAEEAALTCWKTFLHPEGDHFTLISIYKAYQDTTLNSSSEYCVEKWCRDYFLNCSALRMADVIRAELLEIIKRIELPYAEPAFGSKENTLNIKKALLSGYFMQIARDVDGSGNYLMLTHKQVAQLHPLSGYSITKKMPEWVLFHKFSISENNYIRITSEISPELFMQLVPQYYFSNLPPSESKDILQQVVDHLSPVSTMNKEQQMCETCPETEQRCTLQ
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分子量
110.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DHX32 is a member of the DExH box family of RNA helicases, which are critical players in various RNA processing and regulation processes, including splicing, translation, and degradation. Research has revealed that DHX32 is involved in the cellular response to viral infections and the regulation of gene expression, making it a protein of interest for understanding innate immunity and cellular stress responses. Importantly, DHX32 has been implicated in the modulation of the immune response by recognizing viral RNA and facilitating its degradation, thereby helping to limit viral replication. The study of DHX32’s structure and function can provide valuable insights into the mechanisms of RNA metabolism and its links to various diseases, including viral infections and cancer. Moreover, due to its role in regulating key cellular pathways, DHX32 may also serve as a potential therapeutic target. Ongoing investigations into this protein aim to elucidate its biochemical properties, interaction networks, and functional relevance in health and disease, ultimately paving the way for novel strategies in drug development and therapeutic interventions.
