Analytical Data
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基因名
DDX50
- Application
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别名
DDX50; Ddx50; DDX50_HUMAN; DEAD (Asp-Glu-Ala-Asp) box polypeptide 50; DEAD box protein 50; Gu beta; Gu-beta; GU2; GUB; MGC109605; MGC3199; Nucleolar protein Gu2; RH II; RH II/GuB; RNA helicase II / Gu beta
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BQ39
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表达区间
1-737aa
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氨基酸序列
MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEKSETRENGVTDDLDAPKAKKSKMKEKLNGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLDIPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICICFYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHISGASSFEPRSLITSDKGFVTMTLESLEEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNMGVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRSFD
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分子量
109 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DDX50, also known as DDX50 RNA helicase, is a member of the DEAD-box family of RNA helicases, which are essential for various RNA processing events, including transcription, splicing, and translation. The study of DDX50 has gained interest due to its potential roles in cellular stress responses and tumorigenesis. Recent findings suggest that DDX50 may act as a regulator of gene expression by modulating RNA metabolism and stability, impacting processes such as cell proliferation and apoptosis. Its expression levels have been associated with several types of cancers, highlighting DDX50 as a possible biomarker for cancer diagnosis and prognosis. Additionally, the reprogramming of RNA helicases like DDX50 is increasingly recognized as a critical factor in the adaptation of cancer cells to their microenvironment, including stress responses to therapies. Moreover, understanding the mechanistic details of DDX50 action may open new avenues for therapeutic interventions targeting RNA processing pathways. Given the significance of RNA helicases in cellular function, the characterization of DDX50 and its reconstituted protein form serves as a promising area for further research, aiding in the development of cancer treatments and providing insights into the fundamental biology of RNA metabolism. Understanding the structural and functional properties of DDX50 could elucidate its role in normal physiology and pathological conditions, representing a frontier in molecular biology research.
