Analytical Data
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Gene name
DDX4
- Application
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Alternative Names
DDX 4; Ddx4; DDX4_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 4; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MVH; Probable ATP dependent RNA helicase DDX4; Probable ATP-dependent RNA helicase DDX4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NQI0
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Expression Region
1-690aa
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AA Sequence
MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGDNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNKIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD
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Molecular Weight
102.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DDX4, also known as Vasa, is a member of the DEAD-box RNA helicase family and plays a crucial role in germ cell development and spermatogenesis. It is characterized by the presence of a conserved motif that is involved in RNA unwinding and remodeling, which is essential for various cellular processes, including mRNA translation and ribonucleoprotein complex formation. Research on DDX4 has gained significant traction due to its unique expression pattern, predominantly in germ cells, and its implications in reproductive biology and infertility. Abnormalities in DDX4 expression have been linked to various reproductive disorders and germ cell tumors, making it a vital target for investigating the underlying mechanisms of these diseases. Additionally, DDX4 serves as a key marker for the identification of stem cells and primordial germ cells in various organisms, highlighting its potential as a therapeutic target in regenerative medicine and oncology. The recombinant expression of DDX4 allows for the detailed study of its functional properties and interactions with RNA, providing insights into its role in germ cell dynamics. Moreover, understanding the molecular pathways involving DDX4 can contribute to the development of novel strategies for the treatment of infertility and germ cell malignancies. Overall, DDX4's crucial functions in germ cell biology and its association with human diseases underscore the significance of this protein in biomedical research.
