Analytical Data
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基因名
ISL1
- Application
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别名
ISL1；Insulin gene enhancer Protein ISL-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P61371
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表达区间
2-349aa
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氨基酸序列
MASMTGGQQMGRGHHHHHHENLYFQGGEFGDMGDPPKKKRLISLCVGCGN QIHDQYILRVSPDLEWHAACLKCAECNQYLDESCTCFVRDGKTYCKRDYI RLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFAL REDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPISARQPALRP HVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSP RVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPER HDGGLQANPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSN STGSEVASMSSQLPDTPNSMVASPIEAESGGGGSPGRRRRRRRRRRR
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分子量
39 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ISL1 (Islet-1) is a member of the LIM-homeodomain transcription factor family, playing a pivotal role in the development and function of various tissues, particularly in the heart and pancreas. Research into ISL1 has garnered significant interest due to its essential contribution to the differentiation of cardiac progenitor cells and the formation of insulin-producing β-cells in the pancreas. Abnormal expression of ISL1 has been linked to congenital heart defects and diabetes, making it a potential therapeutic target. The study of recombinant ISL1 proteins has emerged as a vital tool in understanding its functional mechanisms and interactions in cellular contexts. By utilizing recombinant DNA technology, researchers can produce large quantities of ISL1 protein for biochemical assays, structural studies, and functional analyses. This has facilitated the exploration of ISL1's role in gene regulation, its interaction with other transcription factors, and its influence on cellular fate decisions. Additionally, recombinant ISL1 proteins are instrumental in investigating the pathways involved in cardiovascular and metabolic diseases, potentially leading to novel therapeutic strategies. Overall, the study of ISL1 recombinant proteins represents a promising avenue for advancing our comprehension of developmental biology and its implications in human health and disease.
