Analytical Data
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Gene name
DDX47
- Application
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Alternative Names
DDX47; DDX47_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 47; DEAD box helicase 47; DEAD box protein 47; DKFZp564O176 ; E4 DBP; FLJ30012 ; HGNC:18682; HQ0256 ; MSTP162
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H0S4
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Expression Region
1-455aa
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AA Sequence
MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR
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Molecular Weight
77 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DDX47 is a member of the DEAD-box protein family, which is characterized by the presence of a conserved RNA helicase core domain. These proteins are known to play crucial roles in various RNA-related processes, including RNA metabolism, splicing, and translation. DDX47 has garnered interest due to its potential involvement in cellular responses to stress, viral replication, and immune responses. Studies have suggested that DDX47 may modulate the interaction between RNA and proteins, thereby influencing gene expression and the stability of RNA molecules. Additionally, its expression patterns and associations with different diseases, including cancers, have prompted investigations into its function and regulatory mechanisms. The development of DDX47 recombinant protein is essential for further studies to elucidate its biological roles and therapeutic potential. By producing and characterizing this protein, researchers can explore its structural properties and interactions with RNA and other biomolecules, ultimately aiding in the understanding of its functions in health and disease contexts. As such, DDX47 represents a promising candidate for future research aimed at developing novel therapeutic strategies targeting RNA regulation and related disorders.
